Effect of the Freezing Step in the Stability and Bioactivity of Protein-Loaded PLGA Nanoparticles Upon Lyophilization

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• 作者：Pedro Fonte ; Fernanda Andrade ; Cláudia Azevedo ; João Pinto…
• 关键词：KEY WORDScryoprotectant ; freezing ; insulin ; lyophilization ; PLGA nanoparticles
• 刊名：Pharmaceutical Research
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：33
• 期：11
• 页码：2777-2793
• 全文大小：2,968 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Pharmacology and Toxicology
  Pharmacy
  Biochemistry
  Medical Law
  Biomedical Engineering
  
• 出版者：Springer Netherlands
• ISSN：1573-904X
• 卷排序：33

文摘

PurposeThe freezing step in lyophilization is the most determinant for the quality of biopharmaceutics. Using insulin as model of therapeutic protein, our aim was to evaluate the freezing effect in the stability and bioactivity of insulin-loaded PLGA nanoparticles. The performance of trehalose, sucrose and sorbitol as cryoprotectants was evaluated.