Seed-Specific Expression of AINTEGUMENTA in Medicago truncatula Led to the Production of Larger Seeds and Improved Seed Germination
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- 作者：Massimo Confalonieri (1)
Maria Carelli (1)
Valentina Galimberti (2)
Anca Macovei (2) (4)
Francesco Panara (3) (5)
Marco Biggiogera (2)
Carla Scotti (1)
Ornella Calderini (3) - 关键词：AINTEGUMENTA ; Cell expansion ; Medicago truncatula ; Seed germination ; Seed size
- 刊名：Plant Molecular Biology Reporter
- 出版年：2014
- 出版时间：October 2014
- 年：2014
- 卷：32
- 期：5
- 页码：957-970
- 全文大小：2,634 KB
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Maria Carelli (1)
Valentina Galimberti (2)
Anca Macovei (2) (4)
Francesco Panara (3) (5)
Marco Biggiogera (2)
Carla Scotti (1)
Ornella Calderini (3)
1. Consiglio per la Ricerca e Sperimentazione in Agricoltura, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, 26900, Lodi, Italy
2. Department of Biology and Biotechnology 鈥淟. Spallanzani鈥? University of Pavia, 27100, Pavia, Italy
4. International Center for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, 110067, New Delhi, India
3. CNR, Istituto di Genetica Vegetale, 06128, Perugia, Italy
5. ENEA Centro Ricerche TRISAIA, 75026, Rotondella, Matera, Italy - ISSN：1572-9818
文摘
The increase of seed size is of great interest in Medicago spp., to improve germination, seedling vigour and, consequently, early forage yield as well as for optimizing seeding techniques and post-seeding management. This study evaluated the effects of the ectopic expression of the AINTEGUMENTA (ANT) cDNA from Arabidopsis thaliana, under the control of the seed-specific USP promoter from Vicia faba, on seed size, germination and seedling growth in barrel medic (Medicago truncatula Gaertn.). All the transgenic T2 barrel medic lines expressing ANT produced seeds significantly larger than those of control plants. Microscopic analysis on transgenic T3 mature seeds revealed that cotyledon storage parenchyma cells were significantly larger and contained larger storage vacuoles than those of the untransformed control. Moreover, the percentage of germination was significantly higher and germination was more rapid in transgenic than in control seeds. Our results indicate that the seed-specific expression of ANT in barrel medic led to larger seeds and improved seed germination, and revealed a regulatory role for ANT in controlling seed size development.