Use of laser interference microscopy to study the state of UV(B)-irradiated mouse peritoneal macrophages

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• 作者：M. V. Zagubizhenko (1)
  A. I. Yusipovich (1)
  S. K. Pirutin (2)
  V. L. Minaev (3) minaev@vniiofi.ru
  Yu. B. Kudryashov (1)
• 关键词：laser interference microscopy (LIM) &#8211 ; macrophages &#8211 ; UV(B) light
• 刊名：Measurement Techniques
• 出版年：2012
• 出版时间：September 2012
• 年：2012
• 卷：55
• 期：6
• 页码：726-730
• 全文大小：150.0 KB
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• 作者单位：1. Lomonosov Moscow State University, Moscow, Russia2. Institute of Theoretical and Experimental Biology, Russian Academy of Sciences, Pushchino, Russia3. All-Russia Research Institute of Optophysical Measurements (VNIIOFI), Moscow, Russia
• ISSN：1573-8906

文摘

We have used laser interference microscopy (LIM) to study damage to the membranes of UV(B)-irradiated mouse peritoneal macrophages. As an indicator of the damage, we used the optical path difference (OPD), proportional to the thickness of the object and the difference between the refractive indices of the object and its surroundings. We compared laser interference microscopy and microfluorimetry for studying the state of the membranes of the irradiated macrophages.