Studies on mitogen-activated protein kinase signaling pathway in the alveolar macrophages of chronic bronchitis rats

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• 作者：Yan Huang ; Xiao-Ming Meng ; Guo-Lin Jiang ; Ya-Ru Yang…
• 关键词：Alveolar macrophages ; Chronic bronchitis ; Mitogen ; activated protein kinase signaling pathway
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：400
• 期：1-2
• 页码：97-105
• 全文大小：968 KB
• 参考文献：1. Song J, Li J, Zheng SR, Jin Y, Huang Y (2013) Anti-inflammatory and immunoregulatory effects of Yupingfeng powder on chronic bronchitis rats. Chin J Integr Med 19(5):353-59. doi:10.1007/s11655-013-1442-6 CrossRef
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  14. Huang Y, Li J, Wang R, Wu Q, Li YH, Yu SC, Cheng WM, Wang YY (2007) Effect of triterpene acids of / Eriobotrya japonica (Thunb.) Lindl. leaf on inflammatory cytokine and mediator induction from alveolar macrophages of chronic bronchitic rats. Inflamm Res 56(2):76-2 CrossRef
  15. Huang Y, Li J, Meng XM, Jiang GL, Li H, Cao Q, Yu SC, Lv XW, Cheng WM (2009) Effect of triterpene acids of / Eriobotrya japonica (Thunb.) Lindl. leaf and MAPK signal transduction pathway on inducible nitric
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

Lipopolysaccharide (LPS), a potent stimulator of inflammatory responses in alveolar macrophages (AMs), activates several intracellular signaling pathways, including mitogen-activated protein kinases (MAPK). In the present study, we investigated the MAPK pathway in AMs of chronic bronchitis (CB) rats. CB was induced by endotracheal instillation of LPS followed by Bacillus Calmette Guerin injection through the caudal vein 1?week later. Specific inhibitors were used and protein phosphorylations were detected by Western blot. We found that Genistein (PTK inhibitor) could inhibit protein kinase C (PKC), phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt or PKB) MAPK signaling pathway with different degrees, LY294002 (PI3K inhibitor) could not only inhibit phospho-PI3K/Akt expression, but also inhibit p38 and c-Jun NH2-terminal kinases (JNK) phosphorylation. Calphostin C (PKC inhibitor) could inhibit phospho-PKC expression and exerted significant effects on extracellular signal-regulated kinases (ERK) phosphorylation, however, it had no impact on p38 and JNK phosphorylation. These results demonstrated that the LPS mediated signaling pathway of MAPK in AMs of CB rats could be described as follows: PTK-PI3K-Akt-JNK/p38 or PTK-PI3K-PKC-ERK, and PI3K may have a negative regulation on the activation of downstream proteins.