Does Japanese medaka (Oryzias latipes) exhibit a gill Na+/K+-ATPase isoform switch during salinity change?

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• 作者：Rebecca J. Bollinger ; Steffen S. Madsen…
• 关键词：α ; Subunit ; Euryhaline teleost ; Na+/K+ ; ATPase enzymatic activity ; Osmoregulation ; Substrate affinity
• 刊名：Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：186
• 期：4
• 页码：485-501
• 全文大小：855 KB
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• 作者单位：Rebecca J. Bollinger (1)
  Steffen S. Madsen (1) (2)
  Maryline C. Bossus (1)
  Christian K. Tipsmark (1)
  
  1. Department of Biological Sciences, University of Arkansas, SCEN 601, Fayetteville, AR, 72701, USA
  2. Department of Biology, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Biomedicine
  Human Physiology
  Zoology
  Animal Physiology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-136X

文摘

Some euryhaline teleosts exhibit a switch in gill Na+/K+-ATPase (Nka) α isoform when moving between fresh water (FW) and seawater (SW). The present study tested the hypothesis that a similar mechanism is present in Japanese medaka and whether salinity affects ouabain, Mg2+, Na+ and K+ affinity of the gill enzyme. Phylogenetic analysis classified six separate medaka Nka α isoforms (α1a, α1b, α1c, α2, α3a and α3b). Medaka acclimated long-term (>30 days) to either FW or SW had similar gill expression of α1c, α2, α3a and α3b, while both α1a and α1b were elevated in SW. Since a potential isoform shift may rely on early changes in transcript abundance, we conducted two short-term (1–3 days) salinity transfer experiments. FW to SW acclimation induced an elevation of α1b and α1a after 1 and 3 days. SW to FW acclimation reduced α1b after 3 days with no other α isoforms affected. To verify that the responses were typical, additional transport proteins were examined. Gill ncc and nhe3 expression were elevated in FW, while cftr and nkcc1a were up-regulated in SW. This is in accordance with putative roles in ion-uptake and secretion. SW-acclimated medaka had higher gill Nka V _max and lower apparent K _m for Na+ compared to FW fish, while apparent affinities for K+, Mg2+ and ouabain were unchanged. The present study showed that the Japanese medaka does not exhibit a salinity-induced α isoform switch and therefore suggests that Na+ affinity changes involve altered posttranslational modification or intermolecular interactions.