Co-inoculation of Urea and DAP Tolerant Sinorhizobium meliloti and Pseudomonas aeruginosa as Integrated Approach for Growth Enhancement of Brassica juncea
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- 作者：Dinesh K. Maheshwari (1)
Sandeep Kumar (1)
Bhavesh Kumar (1)
Piyush Pandey (2) - 关键词：Sinorhizobium meliloti ; Pseudomonas aeruginosa ; Urea ; Diammonium phosphate
- 刊名：Indian Journal of Microbiology
- 出版年：2010
- 出版时间：October 2010
- 年：2010
- 卷：50
- 期：4
- 页码：425-431
- 全文大小：249KB
- 参考文献：1. Sharma R (2004) Use of biofertilizers in rainfed agriculture. Agrobios Newsl 2:16-7
2. Ayala S, Rao EVSP (2002) Perspective of soil fertility management with a focus on fertilizer use for crop productivity. Curr Sci 82:797-07
3. Dekker J (1976) Acquired resistance to fungicides. Ann Rev Phytopathol 14:405-28 CrossRef
4. Cook RJ, Thomoshow LS, Weller DM, Fujimoto D, Mazzola M, Bangera G, Kim D (1995) Molecular mechanism of defense by rhizobacteria against root diseases. Proc Nat Acad Sci 92:4197-201 CrossRef
5. Gupta CP, Dubey RC, Maheshwari DK (2002) Plant growth enhancement and suppression of / Macrophomina / phaseolina causing charcoal rot of peanut by fluorescent / Pseudomonas. Biol Fertil Soils 35:399-05 CrossRef
6. Raj L, Singh VP, Kaushik CD (1997) Stability analysis in crop seed and mustard for resistance to / Alternaria leaf blight and white rust. Ind Phytopathol 50:513-19
7. Yasari E, Esmaeili Azadgoleh MA, Mozafari S, Alashti MR (2009) Enhancement of growth and nutrient uptake of Rapeseed ( / Brassica napus L.) by applying mineral nutrients and biofertilizers. Pak J Biol Sci 12:127-33 CrossRef
8. Vargas MAT, Mandes IC, Hungaria M (2000) Response of field-grown bean ( / Phaseolus vulgaris L.) to / Rhizobium inoculation and nitrogen fertilization in two Carrados soils. Biol Fertil Soils 33:228-33 CrossRef
9. Arora NK, Kang SC, Maheshwari DK (2001) Isolation of siderophore producing strains of / Rhizobium meliloti and their biocontrol potential against / Macrophomina phaseolina that causes charcoal rot of groundnut. Curr Sci 81:673-77
10. Maheshwari DK, Saraf M (1994) Effect of carbaryl and 2, 4-D to nitrogenase and uptake hydrogenase in agar cultures and root nodules formed by / Rhizobium leguminosarum. J Gen Appl Microbiol 40:569-74 CrossRef
11. Saraf M, Sood N (2002) Influence of monocrotophos on growth oxygen uptake and exopolysaccharide production of / Rhizobium NCIM 2771 on chickpea. J Indian Bot Soc 81:154-57
12. Sindhu SS, Gupta SK, Dadarwal KR (1999) Antagonistic effect of / Pseudomonas spp on pathogenic fungi and enhancement of growth of green gram ( / Vigna radiata). Biol Fertil Soils 29:62-8 CrossRef
13. Hewitt EJ (1952) Sand and water culture methods used in the study of plant nutrition. Commonwealth Agricultural Bureaux, Farnham Royal Bucks, p 84
14. Alder JA (1973) A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by / Escherichia / coli. J Gen Microbiol 74:77-1
15. Lopez-de-victoria G, Lowell CR (1993) Chemotaxis of / Azospirillum species to aromatic compounds. Appl Environ Microbiol 59:2951-955
16. Grim AC, Harwood CS (1999) Nay-H, a catabolic plasmid-encoded receptor is required for chemotaxis of / Pseudomonas putida to the aromatic hydrocarbon naphthalene. J Bacteriol 181:3310-316
17. Ahmad A, Khan I, Abdin MZ (2001) Interactive effect of nitrogen and sulphur on nitrogen harvest of rapeseed-mustard. Ind J Plant Physiol 6:46-2
18. Gomez AK, Gomez AA (1984) Statistical procedure for agricultural research. John Wiley and Sons, New York
19. Strzeleowa A (1970) The effect of urea on spheroplast formation in / Rhizobium. Acta Microbiol Pol 2:23-4
20. Maheshwari DK, Gupta M (1991) Diverse effect of two organocarbamets nematicides on nitrogen assimilation of / Rhizobium japonicum 2002 in free-living culture. Biochem Physiol Pflanzen 187:316-22
21. Balasubramaniun A, Palaniappan SP (1983) Effects of two pesticides on oxidative metabolism of cowpea bacteroids. Curr Sci 52:674-75
22. Bhattacharya R, Roy TK (2000) Microbiological and electron microscopic studies of urea treated / Rhizobium sp. Cells Acta Microbiol Pol 49:201-06
23. Pearson JP, Delden C, Iglewski BH (1999) Active efflux and diffusion are involved in transport of / Pseudomonas aeruginosa cell-to-cell signals. J Bacteriol 181:1203-210
24. Fray RG (2002) Altering plant-microbe interaction through artificially manipulating bacterial quorum sensing. Ann Bot 89:245-53 CrossRef
25. Elasri M, Delorme S, Lemanceau P, Stewart G, Laue B, Glickmann E, Oger PM, Dessaux Y (2001) Acyl-homoserine lactone production is more common among plant-associated / Pseudomonas spp. than among soilborne / Pseudomonas spp. App Environ Microbiol 67:1198-209 CrossRef
26. Bashan Y (1998) Inoculants of plant growth promoting bacteria for use in agriculture. Biotechnol Adv 16:729-70 CrossRef
27. Berggren I, Vuurde JWL, Martensson AM (2001) Factor influencing the effect of deleterious / Pseudomonas putida rhizobacteria on initial infection of pea roots by / Rhizobium leguminasorum bv viceae. Appl Soil Ecol 17:97-05 CrossRef
28. Scher FM, Kloepper JW, Singleton C, Zaleska I, Laliberte M (1988) Colonization of soybean roots by / Pseudomonas and / Serratia spp. relationship to bacterial motility, chemotaxis and generation time. Phytopathol 78:1055-059 CrossRef
29. Pandey P, Maheshwari DK (2007) Commensalism between / Sinorhizobium and / Burkholderia in binary-culture consortium for plant growth promotion. Curr Sci 92:1137-142
30. Tripathi RS, Dubey CS, Khan AW, Agarwal KB (1975) Effect of application of / Rhizobium inoculum on yield of gram varieties in chambal command area of Rajasthan. Sci Cult 41:266-69
31. Okon Y (1984) Response of cereal and forage grasses to inoculation with N2- fixing bacteria. In: Veerger C, Newton WE (eds) Advances in nitrogen fixation research. Martinus Nifhoff & Junk Publishers, The Haugue/Wageningen, pp 303-09
32. Mohiuddin MD, Das AK, Ghose DC (2000) Growth and productivity of wheat as influenced by integrated use of chemical fertilizer, biofertilizer and growth regulator. Ind J Plant Physiol 5:334-38 - 作者单位：Dinesh K. Maheshwari (1)
Sandeep Kumar (1)
Bhavesh Kumar (1)
Piyush Pandey (2)
1. Department of Botany and Microbiology, Gurukul Kangri University, Haridwar, 249404, Uttarkhand, India
2. Department of Microbiology, S.B.S.P.G. Institute of Biomedical Sciences and Research, Balawala, Dehradun, 248161, Uttarkhand, India - ISSN：0973-7715
文摘
Two plant growth promoting rhizobacteria–-em class="a-plus-plus">Sinorhizobium meliloti RMP1 and Pseudomonas aeruginosa GRC2 were studied for integrated nutrient management to obtain improved yield of Brassica juncea. Low concentrations of urea and diammonium phosphate (DAP) stimulated the growth of both S. meliloti RMP1 and P. aeruginosa GRC2. 1?M of urea and 0.35?M of DAP was found lethal for RMP1, while 1.3?M and 0.37?M concentrations of urea and DAP proved to be toxic for GRC2. Lc50 was observed as 0.49?M of urea and 0.15?M of DAP for RMP1, and 0.66?M urea and 0.18?M of DAP for GRC2. Urea and DAP adaptive variants of RMP1 and GRC2 was isolated. Adaptive bacterial variants had better growth rates at sub-lethal (Lc50) concentrations of urea and DAP as compared to non-adaptive variants. They also retained plant growth promoting attributes similar to non adaptive variants. GRC2 and RMP1 did not affect the growth of each other and were chemotactically active for DAP, urea as well as root exudates of B. juncea. Both the isolates colonized well in the rhizosphere of B. juncea, as their populations were recorded?? log10 cfu?g? after 120?days. Interestingly, the colonization ability was found even better when both strains were co-inoculated, as their population was recorded in the range of?? log10 cfu?g? after 120?days. In field trials, application of RMP1 and GRC2 resulted in significant increase in biomass and yield of B. juncea as compared to control. However, yield was better with application of half dose and full dose of recommended fertilizers. Interestingly, the biomass as well as yield improved further when both isolates were applied together along with half dose of recommended fertilizers.