Therapeutic effects of high molecular weight hyaluronan injections for tendinopathy in a rat model

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• 作者：Mamoru Yoshida ; Hiroki Funasaki ; Makoto Kubota…
• 刊名：Journal of Orthopaedic Science
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：20
• 期：1
• 页码：186-195
• 全文大小：665 KB
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Orthopedics
  Surgical Orthopedics
  Conservative Orthopedics
  Rheumatology
  Sports Medicine
  
• 出版者：Springer Japan
• ISSN：1436-2023

文摘

Background Tendinopathy is the most common tendon disorder. The etiology is still uncertain, and the disorder poses many therapeutic problems. In a few clinical studies, analgesic effects of high molecular weight hyaluronan (HMW HA) injections were observed, but the underlying mechanisms were not elucidated. In the present study, we analyzed the therapeutic effects of hyaluronan injections for tendinopathy in an animal model. Methods We made the tendinopathy rat model using a rodent treadmill machine. Rats with tendinopathy were injected with HMW HA (HA group), normal saline (NS group), or nothing (control group) into the space between the patellar tendon and the fat pad bilaterally, or were injected with HMW HA into the right knees and with saline to the left knees (HA/NS group), 5 times every 4?days. To assess the pain-relieving effect of HA, the spontaneous locomotor activities at night (12?h) and weight bearing of hind paws were measured every day. Histological sections of the patellar tendon stained with hematoxylin–eosin or prepared by TdT-mediated dUTP nick end labeling were microscopically analyzed. Results The number of spontaneous locomotor activities in the HA group was significantly larger than those in NS or control groups, and in the HA group they recovered up to a healthy level. The percent weight distribution of the right hind paws was significantly increased along with the number of injections. On histologic examinations, the numbers of microtears, laminations, or apoptotic cells in the patellar tendons in the HA group were significantly lower than those in the NS or the control groups. Conclusions The injections of HMW HA were effective for pain relief and for partial restoration of the patellar tendon in our tendinopathy rat model, and thus may become an effective therapeutic modality for the disease.