1H, 13C, 15N resonance assignments of the extracellular loop 1 domain (ECL1) of Streptococcus pneumoniae D39 FtsX, an essential cell

详细信息    查看全文

• 作者：Yue Fu ; Kevin E. Bruce ; Britta Rued ; Malcolm E. Winkler…
• 关键词：Bacterial cell wall ; Divisome ; Peptidoglycan hydrolysis ; ABC transporter ; Allostery ; Extracellular signaling
• 刊名：Biomolecular NMR Assignments
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：10
• 期：1
• 页码：89-92
• 全文大小：598 KB
• 参考文献：Bartual SG, Straume D, Stamsas GA, Munoz IG, Alfonso C, Martinez-Ripoll M, Havarstein LS, Hermoso JA (2014) Structural basis of PcsB-mediated cell separation in Streptococcus pneumoniae. Nat Commun 5:3842
  Bax A, Clore GM, Gronenborn AM (1990) 1H-1H correlation via isotropic mixing of 13C magnetization, a new three-dimensional approach for assigning 1H and 13C spectra of 13C-enriched proteins. J Magn Reson 88(2):425–431ADS
  Delaglio F, Grzesiek S, Vuister G, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277–293CrossRef
  Grzesiek S, Anglister J, Bax A (1993) Correlation of backbone amide and aliphatic side-chain resonances in 13C/15N-enriched proteins by isotropic mixing of 13C magnetization. J Magn Reson 101(1):114–119CrossRef
  Mavrici D, Marakalala MJ, Holton JM, Prigozhin DM, Gee CL, Zhang YJJ, Rubin EJ, Alber T (2014) Mycobacterium tuberculosis FtsX extracellular domain activates the peptidoglycan hydrolase, RipC. Proc Natl Acad Sci USA 111(22):8037–8042ADS CrossRef
  Montelione GT, Lyons BA, Emerson SD, Tashiro M (1992) An efficient triple resonance experiment using carbon-13 isotropic mixing for determining sequence-specific resonance assignments of isotopically-enriched proteins. J Am Chem Soc 114(27):10974–10975CrossRef
  Sham LT, Barendt SM, Kopecky KE, Winkler ME (2011) Essential PcsB putative peptidoglycan hydrolase interacts with the essential FtsX(Spn) cell division protein in Streptococcus pneumoniae D39. Proc Natl Acad Sci USA 108(45):E1061–E1069ADS CrossRef
  Sham LT, Jensen KR, Bruce KE, Winkler ME (2013) Involvement of FtsE ATPase and FtsX extracellular loops 1 and 2 in FtsEX-PcsB complex function in cell division of Streptococcus pneumoniae D39. MBio 4(4):e00431-13. doi:10.​1128/​mBio.​00431-13 CrossRef
  Sheffield P, Garrard S, Derewenda Z (1999) Overcoming expression and purification problems of RhoGDI using a family of “parallel” expression vectors. Protein Expr Purific 15(1):34–39CrossRef
  Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS plus : a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44(4):213–223CrossRef
  
• 作者单位：Yue Fu (1)
  Kevin E. Bruce (2)
  Britta Rued (2)
  Malcolm E. Winkler (2)
  David P. Giedroc (1)
  
  1. Department of Chemistry, Indiana University, 212 S. Hawthorne Drive, Bloomington, IN, 47405-7102, USA
  2. Department of Biology, Indiana University, Bloomington, IN, 47405, USA
  
• 刊物类别：Physics and Astronomy
• 刊物主题：None Assigned
• 出版者：Springer Netherlands
• ISSN：1874-270X

文摘

FtsX is an integral membrane protein from Streptococcus pneumoniae (pneumococcus) that harbors an extracellular loop 1 domain (\({\text{FtsX}}^{\text{ECL1}}_{Spn}\)) that interacts with PcsB, an peptidoglycan hydrolase that is essential for cell growth and division. Here, we report nearly complete backbone and side chain resonance assignments and a secondary structural analysis of \({\text{FtsX}}^{\text{ECL1}}_{Spn}\) (residues 47–168 of FtsX) as first steps toward structure determination of \({\text{FtsX}}^{\text{ECL1}}_{Spn}\).