Functional characterization of Mammary Gland Protein-40, a chitinase-like glycoprotein expressed during mammary gland apoptosis

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• 作者：Vijay Anand ; Shalini Jaswal ; Surender Singh ; Sudarshan Kumar ; Manoj Kumar Jena…
• 关键词：Buffalo Mammary Epithelial cells ; Cytoprotection ; Prolactin ; Stat3 ; Epithelial Mesenchymal Transition
• 刊名：Apoptosis
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：21
• 期：2
• 页码：209-224
• 全文大小：8,587 KB
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• 作者单位：Vijay Anand (1)
  Shalini Jaswal (1)
  Surender Singh (1)
  Sudarshan Kumar (1)
  Manoj Kumar Jena (1)
  Arvind Kumar Verma (1)
  Munna Lal Yadav (1)
  Jagadeesh Janjanam (4)
  Masoud Lotfan (1)
  Dhruba Malakar (1)
  Ajay Kumar Dang (2)
  Tushar Kumar Mohanty (3)
  Jai Kumar Kaushik (1)
  Ashok Kumar Mohanty (1)
  
  1. Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India
  4. Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, 38163, USA
  2. Dairy Cattle Physiology Division, National Dairy Research Institute (NDRI), Karnal, 132001, India
  3. Livestock Production and Management Division, National Dairy Research Institute (NDRI), Karnal, 132001, India
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Cell Biology
  Biochemistry
  Virology
  
• 出版者：Springer Netherlands
• ISSN：1573-675X

文摘

MGP-40 is a chitinase-like protein which is over expressed during mammary gland involution. However, its physiological function in the mammary gland is poorly understood. In the present investigation, we have reported the functional significance of buffalo specific MGP-40 in the mammary gland by using an in vitro model of the buffalo mammary epithelial cell (BuMEC) line. MGP-40 was highly up regulated in BuMECs in serum starved condition as well as after treatment with prolactin suggesting its role in the stress response. Subsequently, to study the effect of MGP-40 on BuMECs, the cells were transfected with a mammalian expression construct of pCI neo harboring MGP-40 gene. It was observed that over expression of MGP-40 enhanced proliferation of BuMECs and protected the cells from apoptosis under serum free condition. In contrast, MGP-40 attenuated the mitogenic effect of insulin in BuMECs. Besides, over expression of the MGP-40 reduced dome formation, acinar polarization and casein synthesis in BuMECs in the presence of lactogenic hormones, it also induced Stat3 phosphorylation and epithelial to mesenchymal transition (EMT) -like features. Together, our data suggest that MGP-40 is involved in protection of BuMECs under stress conditions, inhibits cellular differentiation and induces EMT-like features. A schematic diagram depicting possible association of MGP-40 in various molecular pathways has been presented.