First evidence of Leishmania infection in European brown hare (Lepus europaeus) in Greece: GIS analysis and phylogenetic position within the Leishmania spp
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- 作者：C. N. Tsokana ; C. Sokos ; A. Giannakopoulos ; Z. Mamuris ; P. Birtsas…
- 关键词：Leishmania ; European brown hare ; GIS ; Phylogenetic analysis
- 刊名：Parasitology Research
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：115
- 期：1
- 页码：313-321
- 全文大小：2,589 KB
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C. Sokos (1) (2)
A. Giannakopoulos (1)
Z. Mamuris (3)
P. Birtsas (4)
K. Papaspyropoulos (2)
G. Valiakos (1)
V. Spyrou (5)
M. Lefkaditis (1)
D. C. Chatzopoulos (1)
M. Kantere (1)
K. Manolakou (6)
A. Touloudi (1)
A. Rodi Burriel (1)
E. Ferroglio (7)
C. Hadjichristodoulou (8) (9)
C. Billinis (1)
1. Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece
2. Research Division, Hunting Federation of Macedonia and Thrace, Thessaloniki, Greece
3. Department of Biochemistry & Biotechnology, University of Thessaly, Larissa, Greece
4. Department of Forestry and Natural Environment Administration, Technological Institute of Thessaly, Karditsa, Greece
5. Department of Animal Production, Technological Education Institute of Thessaly, Larissa, Greece
6. Department of Animal Husbandry and Nutrition, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece
7. Department of Veterinary Sciences, University of Turin, Turin, Italy
8. Department of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa, Greece
9. Hellenic Center for Disease Control and Prevention (HCDCP), Ministry of Health, Athens, Greece - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biomedicine
Medical Microbiology
Microbiology
Immunology - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-1955
文摘
Although the existence of a sylvatic transmission cycle of Leishmania spp., independent from the domestic cycle, has been proposed, data are scarce on Leishmania infection in wild mammals in Greece. In this study, we aimed to investigate the presence of Leishmania infection in the European brown hare in Greece, to infer the phylogenetic position of the Leishmania parasites detected in hares in Greece, and to identify any possible correlation between Leishmania infection in hares with environmental parameters, using the geographical information system (GIS). Spleen samples from 166 hares were tested by internal transcribed spacer-1 (ITS-1)-nested PCR for the detection of Leishmania DNA. Phylogenetic analysis was performed on Leishmania sequences from hares in Greece in conjunction with Leishmania sequences from dogs in Greece and 46 Leishmania sequences retrieved from GenBank. The Leishmania DNA prevalence in hares was found to be 23.49 % (95 % confidence interval (CI) 17.27–30.69). The phylogenetic analysis confirmed that the Leishmania sequences from hares in Greece belong in the Leishmania donovani complex. The widespread Leishmania infection in hares should be taken into consideration because under specific circumstances, this species can act as a reservoir host. This study suggests that the role of wild animals, including hares, in the epidemiology of Leishmania spp. in Greece deserves further elucidation.