Functional analysis of the calcineurin-encoding gene cnaA from Aspergillus oryzae: evidence for its putative role in stress adaptation

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• 作者：Praveen Rao Juvvadi ; Yutaka Kuroki ; Manabu Arioka ; Harushi Nakajima and Katsuhiko Kitamoto
• 刊名：Archives of Microbiology
• 出版年：2003
• 出版时间：June 2003
• 年：2003
• 卷：179
• 期：6
• 页码：416-422
• 全文大小：376 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X

文摘

The presence of putative STRE (stress response regulatory element) and HSF (heat-shock factor) transcription factor binding sites in the promoter region of the gene encoding calcineurin (cnaA) from Aspergillus oryzae implicated a probable role for calcineurin in the stress response. The activity of calcineurin was enhanced during growth of the wild-type strain in the presence of 1 M NaCl (2.6-fold), at alkaline pH 10.0 (2.9-fold) and at 37 °C (1.6-fold). The induction of cnaA antisense expression resulted in reduced calcineurin activity (1.4-fold) and caused a growth defect under the stress conditions. Induction of a strain overexpressing cnaA resulted in an increase in calcineurin activity under stress conditions, such as the presence of 1 M NaCl (73%), alkaline pH 10.0 (70%), and a temperature of 37 °C (50%), in addition to tolerance to FK506 (a specific inhibitor of calcineurin). While a role for calcineurin in hyphal growth is well recognized, the present study suggests that stress adaptation mechanisms in filamentous fungi involve calmodulin/calcineurin-mediated signal transduction pathways.