α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

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• 作者：Asha Rani ; Subhadeep Roy ; Manjari Singh ; Uma Devi ; Rajnish K. Yadav…
• 关键词：α ; Chymotrypsin ; Mammary gland ; PGP 9.5 ; Oxidative stress ; NF ; κB p65 ; FAME
• 刊名：Inflammopharmacology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：24
• 期：5
• 页码：277-286
• 全文大小：971 KB
• 刊物主题：Pharmacology/Toxicology; Immunology; Rheumatology; Gastroenterology; Dermatology; Allergology;
• 出版者：Springer Basel
• ISSN：1568-5608
• 卷排序：24

文摘

This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 % normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.