Identification of microorganisms by FTIR spectroscopy: perspectives and limitations of the method
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  • 作者:Mareike Wenning (1)
    Siegfried Scherer (1) (2)
  • 关键词:FTIR spectroscopy ; Species identification ; Strain typing ; Chemometrics
  • 刊名:Applied Microbiology and Biotechnology
  • 出版年:2013
  • 出版时间:August 2013
  • 年:2013
  • 卷:97
  • 期:16
  • 页码:7111-7120
  • 全文大小:233KB
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  • 作者单位:Mareike Wenning (1)
    Siegfried Scherer (1) (2)

    1. Abteilung Mikrobiologie, Zentralinstitut f眉r Ern盲hrungs- und Lebensmittelforschung (ZIEL), Technische Universit盲t M眉nchen, Weihenstephaner Berg 3, D-85350, Freising, Germany
    2. Lehrstuhl f眉r Mikrobielle 脰kologie, Department of Biosciences, Technische Universit盲t M眉nchen, D-85350, Freising, Germany
文摘
Fourier transform infrared (FTIR) spectroscopy was introduced in 1991 as a technique to identify and classify microbes. Since then, it has gained growing interest and has undergone a remarkable evolution. Highly sophisticated spectrometers have been developed, enabling a high sample throughput. Today, the generation of high-quality data in a short time and the application of the technique for rapid and reliable identification of microbes to the species level are well documented. What makes FTIR spectroscopy even more attractive is the fact that spectral information can also be exploited for strain typing purposes, which is particularly important for epidemiological analyses and some technological applications. Accordingly, in recent years, FTIR spectroscopy has been increasingly used for typing and classifying microorganisms below the species level. The intention of this review is to give an overview over current knowledge and strategies of using FTIR spectroscopy for species identification and to describe different approaches for strain typing.

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