Forage preservation (grazing vs. hay) fed to ewes affects the fatty acid profile of milk and CPT1B gene expression in the sheep mammary gland

详细信息    查看全文

• 作者：Elda Dervishi (1)
  Margalida Joy (1)
  Albina Sanz (1)
  Javier Alvarez-Rodriguez (2)
  Francisco Molino (1)
  Jorge H Calvo (1) (3)
  
• 刊名：BMC Veterinary Research
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：8
• 期：1
• 全文大小：297KB
• 参考文献：1. Chilliard YA, Ferlay R, Mansbridge M, Doreau M: Ruminant milk fat plasticity: nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. / Ann Zootech 2000, 49:181鈥?05. CrossRef
  2. Williams CM: Dietary fatty acids and human health. / Ann Zootech 2000, 49:165鈥?80. CrossRef
  3. Massaro M, Carluccio MA, de Caterina R: Direct vascular antiatherogenic effects of oleic acid: a clue to the cardioprotective effects of the Mediterranean diet. / Cardiologia 1999, 44:507鈥?13.
  4. Pariza MW, Park Y, Cook ME: The biologically active isomers of conjugated linoleic acid. / Prog Lipid Res 2001, 40:283鈥?98. CrossRef
  5. Chilliard Y, Ferlay A, Doreau M: Effect of different types of forages, animal fat or marine oils in cow's diet on milk fat secretion and composition, especially conjugated linoleic acid (CLA) and polyunsaturated fatty acids. / Livest Prod Sci 2001, 70:31鈥?8. CrossRef
  6. Chilliard Y, Ferlay A: Dietary lipids and forage interactions on cow and goat milk fatty acid composition and sensory properties. / Reprod Nut Devel 2004, 44:467鈥?92. CrossRef
  7. Scerra M, Caparra P, Foti F, Galofaro V, Sinatra MC, Scerra V: Influence of ewe feeding systems on fatty acid composition of suckling lambs. / Meat Sci 2007, 76:390鈥?94. CrossRef
  8. Joy M, Alvarez-Rodriguez J, Revilla R, Delfa R, Ripoll G: Ewe metabolic performance and lamb carcass traits in pasture and concentrate-based production systems in Churra Tensina breed. / Small Rumin Res 2008, 75:24鈥?5. CrossRef
  9. Joy M, Ripoll R, Molino F, Dervishi E, Alvarez-Rodriguez J: Influence of the type of forage supplied to ewes in pre- and post-partum periods on the meat fatty acids of suckling lambs. / Meat Sci 2012, 90:775鈥?82. CrossRef
  10. Tsiplakou E, Mountzouris KC, Zervas G: Concentration of conjugated linoleic acid in grazing sheep and goat milk fat. / Livest Sci 2006, 103:74鈥?4. CrossRef
  11. Bernard L, Leroux C, Faulconnier Y, Durand D, Shingfield KJ, Chilliard Y: Effect of sunflower-seed oil or linseed oil on milk fatty acid secretion and lipogenic gene expression in goats fed hay-based diets. / J Dairy Res 2009, 76:241鈥?48. CrossRef
  12. Bionaz M, Loor JJ: Gene networks driving bovine milk fat synthesis during the lactation cycle. / BMC Genomics 2008, 9:366. CrossRef
  13. Tsiplakou E, Flemetakis E, Kalloniati C, Papadomichelakis G, Katinakis P, Zervas G: Sheep and goats differences in CLA and fatty acids milk fat content in relation with mRNA stearoyl-CoA desaturase and lipogenic genes expression in their mammary gland. / J Dairy Res 2009,76(4):392鈥?01. CrossRef
  14. Tsiplakou E, Flemetakis E, Kalloniati C, Zervas G: Differences in mRNA lipogenic gene expression in the subcutaneous adipose tissue of sheep and goats under the same dietary treatments. / Small Rumin Res 2011,99(2鈥?):110鈥?15. CrossRef
  15. Tsiplakou E, Kominakis A, Zervas G: The interaction between breed and diet on CLA and fatty acids content of milk fat of four sheep breeds kept indoors or at grass. / Small Rumin Res 2008,74(1鈥?):179鈥?87. CrossRef
  16. Dervishi E, Serrano C, Joy M, Serrano M, Rodellar C, Calvo JH: The effect of feeding system in the expression of genes related with fat metabolism in semitendinous muscle in sheep. / Meat Sci 2011, 89:91鈥?7. CrossRef
  17. Reverter A, Byrne KA, Bruce HL, Wang YH, Dalrymple BP, Lehnert SA: A mixture model- based cluster analysis of DNA microarray gene expression data on Brahman and Brahman composite steers fed high-, medium-, and lowquality diets. / J Anim Sci 2003, 81:1900鈥?910.
  18. Doreau M, Poncet C: Ruminal biohydrogenation of fatty acids originating from fresh or preserved grass. / Reprod Nutr Dev 2000, 40:201.
  19. Valvo MA, Lanza M, Bella M, Fasone V, Scerra M, Biondi L: Effect of ewe feeding system (grass v. concentrate) on intramuscular fatty acids of lambs raised exclusively on maternal milk. / Anim Sci 2005, 81:431鈥?36. CrossRef
  20. Russel AJF, Doney JM, Gunn RG: Subjective assessment of body fat in live sheep. / J Agr Sci 1969, 72:451鈥?54. CrossRef
  21. Alvarez-Rodriguez J, Sanz A, Delfa R, Revilla R, Joy M: Performance and grazing behaviour of Churra Tensina sheep stocked under different management systems during lactation on Spanish mountain pastures. / Livest Sci 2007, 107:152鈥?61. CrossRef
  22. Doney JM, Peart JN, Smith WF, Louda F: Consideration of the techniques for estimation of milk-yield by suckled sheep and a comparison of estimates obtained by 2 methods in relation to the effect of breed, level of production and stage of lactation. / J Agr Sci 1979, 92:123鈥?32. CrossRef
  23. European Union (EU) Directive No. 86/609/CEE: Council Directive of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. / Offic J Europ Commun 1986,1986(358):1鈥?2.
  24. Sukhija PS, Palmquist DL: Rapid method for determination of total fatty acid content and composition of feedstuffs and faeces. / J Agric Food Chem 1988, 36:1202鈥?206. CrossRef
  25. Luna P, Ju谩rez M, de la Fuente MA: Validation of rapid milk fat separation method to determine the fatty acid profile by gas cromatography. / J Anim Sci 2005, 88:3377鈥?381.
  26. BOE: Aceites y grasas de origen animal y vegetal. Preparaci贸n de 茅steres met铆licos de 谩cidos grasos. / Resoluci贸n de 7 de mayo de 2001, de la Direcci贸n General de Pol铆tica Tecnol贸gica, por la que se publica la relaci贸n de normas UNE aprobadas por AENOR durante el mes de febrero de 2001 2001, 136:20223鈥?0227.
  27. De La Fuente LF, Barbosa E, Carriedo JA, Gonzalo C, Arenas R, Fresno JM, San Primitivo F: Factors influencing variation of fatty acid content in ovine milk. / J Dairy Sci 2009, 92:3791鈥?799. CrossRef
  28. Larinov A, Krause A, Miller W: A standard curve based method for relative real time PCR data processing. / BMC Bioinformatics 2005, 6:62. CrossRef
  29. Andersen CL, Jensen JL, Orntoft TF: Normalization of Real- Time quantitative reverse transcription- PCR data: A model- based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data set. / Cancer Res 2004, 64:5245鈥?250. CrossRef
  30. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. / Genome Biol 2002,3(7):0034. CrossRef
  31. Cannas A, Tedeschi LO, Fox DG, Pell AN, Van Soest PJ: A mechanistic model for predicting the nutrient requirements and feed biological values for sheep. / J Anim Sci 2004, 82:149鈥?69.
  32. INRA: / Alimentation des bovins, ovins et caprins. Besoin des animaux, valeurs des aliments. Quae Editions edition. Versailles, France; 2007.
  33. Biondi L, Valvo MA, Di Gloria M, Tenghi ES, Galofaro V, Priolo A: Changes in ewe milk fatty acids following turning out to pasture. / Small Rumin Res 2008, 75:17鈥?3. CrossRef
  34. Dewhurst RJ, Shingfield KJ, Lee MRF, Scollan ND: Increasing the concentrations of beneficial polyunsaturated fatty acids in milk produced by dairy cows in high-forage systems. / Anim Feed Sci Tech 2006,131(3鈥?):168鈥?06. CrossRef
  35. Woods VB, Fearon AM: Dietary sources of unsaturated fatty acids for animals and their transfer into meat, milk and eggs: A review. / Livest Sci 2009,126(1鈥?):1鈥?0. CrossRef
  36. Cabiddu A, Decandia M, Addis M, Piredda G, Pirisi A, Molle G: Managing Mediterranean pastures in order to enhance the level of beneficial fatty acids in sheep milk. / Small Rumin Res 2005,59(2鈥?):169鈥?80. CrossRef
  37. Atti N, Rouissi H, Othmane MH: Milk production, milk fatty acid composition and conjugated linoleic acid (CLA) content in dairy ewes raised on feedlot or grazing pasture. / Livest Sci 2006, 104:121鈥?27. CrossRef
  38. Nudda A, McGuire MA, Battacone G, Pulina G: Seasonal variation inconjugated linoleic acid and vaccenic acid in milk fat of sheep and its transfer to cheese and ricotta. / J Dairy Sci 2005, 88:1311鈥?319. CrossRef
  39. McGarry JD, Woeltje KF, Kuwajima M, Foster DW: Regulation of ketogenesis and the renaissance of carnitine palmitoyltransferase. / Diabetes Metab Rev 1989, 5:271鈥?84. CrossRef
  40. Girard J, Chatelain F, Boillot J, Prip-Buus C, Thumelin S, P茅gorier JP, Foufelle F, Ferr P: Nutrient regulation of gene expression. / J Anim Sci 1997, 75:46鈥?7.
  41. Yu GS, Lu YC, Gulick T: Co-regulation of tissue-specific alternative human carnitine palmitoyltransferase I尾 gene promoters by fatty acid enzyme substrate. / J Biol Chem 1998, 273:32901鈥?2909. CrossRef
  42. Brandt JM, Djouadi F, Kelly DP: Fatty acids activate transcription of muscle carnitine palmitoyltransferase 1 gene in cardiac myocytes via peroxisome proliferator activated receptor alpha. / J Biol Chem 1998, 273:23786鈥?3792. CrossRef
  43. Moore ML, Wang GL, Belaguli NS, Schwartz RJ, McMillin JB: GATA-4 and serum response factor regulate transcription of the muscle-specific carnitine palmitoyltransferase I beta in rat heart. / J Biol Chem 2001, 276:1026鈥?033. CrossRef
  44. Steffen ML, Harrison WR, Elder FF, Cook GA, Park EA: Expression of the rat liver carnitine palmitoyltransferase I (CPT-Ialpha) gene is regulated by Sp1 and nuclear factor Y: chromosomal localization and promoter characterization. / Biochem J 1999, 340:425鈥?32. CrossRef
  45. Djouadi F, Brandt JM, Weinheimer CJ, Leone TC, Gonzalez FJ, Kelly DP: The role of the peroxisome proliferator-activated receptor alpha (PPAR alpha) in the control of cardiac lipid metabolism. / Prostaglandins Leukotrienes Essent Fatty Acids 1999, 60:339鈥?43鈥?43鈥?60. CrossRef
  46. McGarry JD, Leatherman GF, Foster DW: Carnitine palmitoyltransferase I: the site of inhibition of hepatic fatty acid oxidation by malonyl-CoA. / J Biol Chem 1978, 253:4128鈥?136.
  47. Kerner J, Hoppel C: Fatty acid import into mitochondria. / Biochim Biophys Acta 2000, 1486:1鈥?7. CrossRef
  48. Rasmussen BB, Winder WW: Effect of exercise intensity on skeletal muscle malonyl- CoA and acetyl- CoA carboxylase. / Am Physiol Soc 1997, 83:1104鈥?109.
  49. Bonnefont JP, Djouadi F, Prip-Buus C, Gobin S, Munnich A, Bastin J: Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects. / Mol Aspects Med 2004, 25:495鈥?20. CrossRef
  50. Shi X, Liu S, Metges CC, Seyfert HM: C/EBP-beta drives expression of the nutritionally regulated promoter IA of the acetyl-CoA carboxylase-alpha gene in cattle. / Biochim Biophys Acta 2010, 1799:561鈥?67. CrossRef
  51. Harvatine KJ, Bauman DE: SREBP1 and Thyroid Hormone Responsive Spot 14 (S14) are involved in the regulation of bovine mammary lipid synthesis during diet-induced milk fat depression and treatment with CLA. / J Nutr Biochem 2006,136(10):2468鈥?473.
  
• 作者单位：Elda Dervishi (1)
  Margalida Joy (1)
  Albina Sanz (1)
  Javier Alvarez-Rodriguez (2)
  Francisco Molino (1)
  Jorge H Calvo (1) (3)
  
  1. Unidad de Tecnolog铆a en Producci贸n Animal, CITA, Zaragoza, Spain
  2. Departament de Producci贸 Animal, Universitat de Lleida, Lleida, Spain
  3. Fundaci贸n ARAID, Zaragoza, Spain
  

文摘

Background Alterations in lipid metabolism occur when animals are exposed to different feeding systems. In the last few decades, the characterisation of genes involved in fat metabolism and technological advances have enabled the study of the effect of diet on the milk fatty acid (FA) profile in the mammary gland and aided in the elucidation of the mechanisms of the response to diet. The aim of this study was to evaluate the effect of different forage diets (grazing vs. hay) near the time of ewe parturition on the relationship between the fatty acid profile and gene expression in the mammary gland of the Churra Tensina sheep breed. Results In this study, the forage type affected the C18:2 cis-9 trans-11 (CLA) and long-chain saturated fatty acid (LCFA) content, with higher percentages during grazing than during hay feeding. This may suggest that these FAs act as regulatory factors for the transcriptional control of the carnitine palmitoyltransferase 1B (CPT1B) gene, which was more highly expressed in the grazing group (GRE). The most highly expressed gene in the mammary gland at the fifth week of lactation is CAAT/ enhancer- binding protein beta (CEBPB), possibly due to its role in milk fat synthesis in the mammary gland. More stable housekeeping genes in the ovine mammary gland that would be appropriate for use in gene expression studies were ribosomal protein L19 (RPL19) and glyceraldehyde- 3- phosphate dehydrogenase (GAPDH). Conclusions Small changes in diet, such as the forage preservation (grazing vs. hay), can affect the milk fatty acid profile and the expression of the CPT1B gene, which is associated with the oxidation of fatty acids. When compared to hay fed indoors, grazing fresh low mountain pastures stimulates the milk content of CLA and LCFA via mammary uptake. In this sense, LCFA in milk may be acting as a regulatory factor for transcriptional control of the CPT1B gene, which was more highly expressed in the grazing group.