Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
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- 作者：Marta Bencke-Malato (1)
Caroline Cabreira (1)
Beatriz Wiebke-Strohm (1)
Lauro B眉cker-Neto (1)
Estefania Mancini (2)
Marina B Osorio (1)
Milena S Homrich (1)
Andreia Carina Turchetto-Zolet (1)
Mayra CCG De Carvalho (3)
Renata Stolf (3)
Ricardo LM Weber (1)
Gast贸n Westergaard (2)
At铆lio P Castagnaro (4)
Ricardo V Abdelnoor (3)
Francismar C Marcelino-Guimar茫es (3)
M谩rcia Margis-Pinheiro (1)
Maria Helena Bodanese-Zanettini (1)
1. Programa de P贸s-Gradua莽茫o em Gen茅tica e Biologia Molecular ; Universidade Federal do Rio Grande do Sul (UFRGS) ; Porto Alegre ; Brazil
2. Instituto de Agrobiotecnologia Rosario SA ; Rosario ; Argentina
3. Empresa Brasileira de Pesquisa Agropecu谩ria (Embrapa Soja) ; Londrina ; Brazil
4. Estaci贸n Experimental Agroindustrial Obispo Colombres (EEAOC) ; Tucum谩n ; Argentina - 关键词：Glycine max ; Genetic transformation ; Fungus resistance ; Transcription factors ; Asian Soybean Rust ; Functional analysis
- 刊名：BMC Plant Biology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：3,182 KB
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- 出版者：BioMed Central
- ISSN：1471-2229
文摘
Background Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.