Magnetomotive Optical Coherence Tomography for the Assessment of Atherosclerotic Lesions Using αvβ3 Integrin-Targeted Microspheres

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• 作者：Jongsik Kim (1)
  Adeel Ahmad (2)
  Marina Marjanovic (1) (3)
  Eric J. Chaney (1)
  Joanne Li (4)
  Jonathan Rasio (5)
  Zita Hubler (3)
  Darold Spillman (1)
  Kenneth S. Suslick (1) (6)
  Stephen A. Boppart (1) (2) (3) (7)
  
• 关键词：Optical coherence tomography ; Magnetomotive ; Protein microspheres ; Fatty streaks ; Atherosclerosis ; αvβ3 integrin ; Flow chamber
• 刊名：Molecular Imaging and Biology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：16
• 期：1
• 页码：36-43
• 全文大小：842 KB
• 作者单位：Jongsik Kim (1)
  Adeel Ahmad (2)
  Marina Marjanovic (1) (3)
  Eric J. Chaney (1)
  Joanne Li (4)
  Jonathan Rasio (5)
  Zita Hubler (3)
  Darold Spillman (1)
  Kenneth S. Suslick (1) (6)
  Stephen A. Boppart (1) (2) (3) (7)
  
  1. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, IL, 61801, USA
  2. Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 1406 West Green Street, Urbana, IL, 61801, USA
  3. Department of Bioengineering, University of Illinois at Urbana-Champaign, 1304 West Springfield Avenue, Urbana, IL, 61801, USA
  4. Department of Nuclear, Plasma and Radiological Engineering, University of Illinois at Urbana-Champaign, 104 South Wright Street, Urbana, IL, 61801, USA
  5. Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL, 61801, USA
  6. Department of Chemistry, University of Illinois at Urbana-Champaign, 505 South Mathews Avenue, Urbana, IL, 61801, USA
  7. Department of Internal Medicine, University of Illinois at Urbana-Champaign, 506 South Mathews Avenue, Urbana, IL, 61801, USA
  
• ISSN：1860-2002

文摘

Purpose We investigated the early-stage fatty streaks/plaques detection using magnetomotive optical coherence tomography (MM-OCT) in conjunction with αvβ3 integrin-targeted magnetic microspheres (MSs). The targeting of functionalized MSs was investigated by perfusing ex vivo aortas from an atherosclerotic rabbit model in a custom-designed flow chamber at physiologically relevant pulsatile flow rates and pressures. Procedures Aortas were extracted and placed in a flow chamber. Magnetic MS contrast agents were perfused through the aortas and MM-OCT, fluorescence confocal, and bright field microscopy were performed on the ex vivo aorta specimens for localizing the MSs. Results The results showed a statistically significant and stronger MM-OCT signal (3.30?±-.73?dB) from the aorta segment perfused with targeted MSs, compared with the nontargeted MSs (1.18?±-.94?dB) and control (0.78?±-.41?dB) aortas. In addition, there was a good co-registration of MM-OCT signals with confocal microscopy. Conclusions Early-stage fatty streaks/plaques have been successfully detected using MM-OCT in conjunction with αvβ3 integrin-targeted magnetic MSs.