A NMR experiment for simultaneous correlations of valine and leucine/isoleucine methyls with carbonyl chemical shifts in proteins

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• 作者：Vitali Tugarinov (1)
  Vincenzo Venditti (1)
  G. Marius Clore (1)
  
• 关键词：Chemical shift assignments ; Methyl labeling ; Enzyme I ; Methyl TROSY ; Isotope shifts
• 刊名：Journal of Biomolecular NMR
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：58
• 期：1
• 页码：1-8
• 全文大小：1,079 KB
• 作者单位：Vitali Tugarinov (1)
  Vincenzo Venditti (1)
  G. Marius Clore (1)
  
  1. Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0520, USA
  
• ISSN：1573-5001

文摘

A methyl-detected ‘out-and-back-NMR experiment for obtaining simultaneous correlations of methyl resonances of valine and isoleucine/leucine residues with backbone carbonyl chemical shifts, SIM-HMCM(CGCBCA)CO, is described. The developed pulse-scheme serves the purpose of convenience in recording a single data set for all Ileδ1, Leuδ and Valγ (ILV) methyl positions instead of acquiring two separate spectra selective for valine or leucine/isoleucine residues. The SIM-HMCM(CGCBCA)CO experiment can be used for ILV methyl assignments in moderately sized protein systems (up to ~100?kDa) where the backbone chemical shifts of 13Cα, 13Cβ and 13CO are known from prior NMR studies and where some losses in sensitivity can be tolerated for the sake of an overall reduction in NMR acquisition time.