Bone marrow micrometastases in 109 breast cancer patients:Correlations with clinical and pathological features and prognosis

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• 作者：Molino ; Annamaria ; Pelosi ; Giuseppe ; Turazza ; Monica ; Sperotto ; Loris ; Bonetti ; Andrea ; Nortilli ; Rolando ; Fattovich ; Giovanni ; Alaimo ; Cristoforo ; Piubello ; Quirino ; Pavanel ; Flavia ; Micciolo ; Rocco ; Cetto ; Gian Luigi
• 关键词：bone marrow ; breast cancer ; micrometastases ; prognosis
• 刊名：Breast Cancer Research and Treatment
• 出版年：1997
• 出版时间：1997
• 年：1997
• 卷：42
• 期：1
• 页码：23-30
• 全文大小：40 KB

文摘

Background: The presence in bone marrow of cellswhich react with monoclonal antibodies against tumor-associated antigenshas been proposed over the last few yearsas a new prognostic factor in breast cancer patients.Patients and methods: Bone marrow aspirates were obtainedfrom 109 stage I and II breast cancerpatients during or 2–4 weeks after primary surgery.The samples were processed for leukocyte separation ona Ficoll-Hypaque gradient and then used to preparecytospin slides for immunocytochemical analysis. The slides werestained with a pool of monoclonal antibodies (MoAbs)which recognize tumor associated antigens, using the alkalinephosphatase anti-alkaline phosphatase method. The median follow-up was36 months (range 15–62); 22 patients relapsed and7 died.Results: Thirty-four of the 109 patients (31.1%) hadMoAb positive bone marrow cells. The bone marrowwas positive in 28/74 (37.9%) patients who hadthe aspirate taken during surgery and in 6/35(17.1%) who had it taken after surgery (p = 0.055).No association was found between bone marrow positivity and tumour size,nodal status, menopausal status, estrogen receptor positivityor the proliferative index. No association was found between bone marrow andprognosis: the log-rank test was 0.291 (p >0.5) for OS and 0.023 for DFS; thehazard ratio (positive vs negative) was 1.51 forOS (95% CI: 0.33–6.86) and 0.93 for DFS(95% CI: 0.35–2.45).Conclusions: In our series, bone marrow positivity didnot correlate with prognostic parameters or prognosis. Ofinterest is the relative excess of positivity whenthe bone marrow was obtained during surgery.