Cell-type-specific expression pattern of ceramide synthase 2 protein in mouse tissues

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• 作者：Christiane Kremser ; Anna-Lena Klemm ; Martina van Uelft…
• 关键词：Brain ; Ceramide synthase ; Myelin ; Oligodendrocytes ; Sphingolipid
• 刊名：Histochemistry and Cell Biology
• 出版年：2013
• 出版时间：November 2013
• 年：2013
• 卷：140
• 期：5
• 页码：533-547
• 全文大小：1770KB
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• 作者单位：Christiane Kremser (1)
  Anna-Lena Klemm (1)
  Martina van Uelft (1)
  Silke Imgrund (1)
  Christina Ginkel (1)
  Dieter Hartmann (2)
  Klaus Willecke (1)
  
  1. Molecular Genetics, Life and Medical Sciences (LIMES)-Institute, University of Bonn, Carl-Troll-Str. 31, 53115, Bonn, Germany
  2. Division of Neuroanatomy, Department of Anatomy, University of Bonn, 53115, Bonn, Germany
  
• ISSN：1432-119X

文摘

Ceramide synthase 2 (CerS2) catalyzes the synthesis of dihydroceramides from dihydrosphingosine and very long fatty acyl (C22–C24)-CoAs. CerS2-deficient (gene trap) mice were reported to exhibit myelin and behavioral abnormalities, associated with the expression of CerS2 in oligodendrocytes and neurons based on expression of lacZ reporter cDNA instead of the cers2 gene in these mice. In order to clarify the cell-type-specific expression of CerS2 protein, we have raised antibodies that specifically recognize the glycosylated and non-glycosylated CerS2 protein in wild-type but not in CerS2-deficient mouse tissues. In early postnatal, juvenile and adult mouse brain, the new antibodies detect CerS2 protein only in oligodendrocytes but not in neurons, suggesting that the gene trap vector in CerS2-deficient mice led to ectopic expression of the lacZ reporter gene in neurons. In liver, the CerS2 protein is expressed in hepatocytes but not in Ito cells or Kupffer cells. We conclude that the behavioral abnormalities observed in CerS2-deficient mice originate primarily in oligodendrocytes and not in neurons. The identification of specific cell types in which CerS2 protein is expressed is prerequisite to further mechanistic characterization of phenotypic abnormalities exhibited by CerS2-deficient mice. The amount of CerS2 protein detected in different tissues by immunoblot analyses does not strictly correspond to the activity of the CerS2 enzyme. Disproportional results are likely due to post-translational regulation of the CerS2 protein.