Screening of microalgae for primary metabolites including β-glucans and the influence of nitrate starvation and irradiance on β-glucan production
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- 作者：Christian Schulze ; Martin Wetzel ; Jakob Reinhardt…
- 关键词：β ; glucan ; Lipids ; Microalgae ; Nitrate starvation ; Proteins ; Scenedesmus
- 刊名：Journal of Applied Phycology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：28
- 期：5
- 页码：2719-2725
- 全文大小：424 KB
- 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
- 出版者：Springer Netherlands
- ISSN：1573-5176
- 卷排序：28
文摘
Βeta-glucans, widespread glucose polymers in mushrooms, yeasts, and bacteria, but rarely found in microalgae, have wide applications and high medicinal and economical potential. Some β-glucans like paramylon from algae-like Euglena gracilis are well investigated, but there is little information about the β-glucan content of microalgae. Therefore, more than 40 species of cultured microalgae have been investigated for composition of their biomass regarding to lipids, carbohydrates including β-glucans and proteins. Most of algae species showed a rather similar biomass composition of about 10 % lipids, 25 % carbohydrates, and 40 % proteins if they have been cultivated in a full medium, rather low light conditions of 50 μmol photons m−2 s−1, 12/12 h light/dark cycle under aeration and a temperature of 25 ± 2 °C. The content of β-glucans varied between 1.7 and 24.2 % of dry weight, respectively. Two microalgae, Scenedesmus ovalternus SAG 52.80 and Porphyridium purpureum SAG 1380-1d with a yield of more than 20 % of dry weight were identified as the best β-glucan producers under standard cultivation conditions. Culture optimization experiments revealed that enhanced irradiance increased the β-glucan content of Scenedesmus obtusiusculus A 189, a novel green algae isolate, from 6.4 to 19.5 %, but the β-glucan content of the green alga S. ovalternus SAG 52.80 remained unaffected (24.2 vs. 23.3 %). Nitrate starvation enhanced the β-glucan content of S. obtusiusculus A 189 from 16 to 23 % and of S. ovalternus SAG 52.80 from 23.3 to 36.7 %.