Impaired liver function in Xenopus tropicalis exposed to benzo[a]pyrene: transcriptomic and metabolic evidence
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- 作者：Christophe Regnault (11) (12) (13)
Isabelle AM Worms (14)
Christine Oger-Desfeux (15)
Christelle MelodeLima (11) (12)
Sylvie Veyrenc (11) (12) (13)
Marie-Laure Bayle (16)
Bruno Combourieu (16)
Aur茅lie Bonin (11) (12)
Julien Renaud (11) (12)
Muriel Raveton (11) (12) (13)
St茅phane Reynaud (11) (12) (13) (17)
11. University Grenoble Alpes ; LECA ; F-38000 ; Grenoble ; France
12. CNRS ; LECA ; F-38000 ; Grenoble ; France
13. University Grenoble Alpes ; BEeZy ; F-38000 ; Grenoble ; France
14. Universit茅 de Gen猫ve ; Institute F.A. Forel ; Versoix ; Suisse
15. P么le Rh么ne Alpes de Bioinformatique ; Universit茅 Claude Bernard Lyon 1 ; Villeurbanne ; France
16. Plateforme de recherche en toxicologie environnementale et 茅cotoxicologie de Rovaltain ; Valence ; France
17. Laboratoire d鈥橢cologie Alpine (LECA) ; UMR CNRS-Universit茅 de Grenoble 5553 ; Domaine Universitaire de Saint-Martin d鈥橦猫res ; 2233 ; rue de la piscine B芒t D Biologie ; BP 53 ; 38041 ; Grenoble Cedex 9 ; France - 关键词：RNA sequencing ; Xenopus ; Liver ; Metabolic disorders ; Insulin resistance ; like syndrome ; Benzo[a]pyrene
- 刊名：BMC Genomics
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：15
- 期：1
- 全文大小：2,258 KB
- 参考文献：1. Lips, KR, Diffendorfer, J, Mendelson, JR, Sears, MW (2008) Riding the wave: Reconciling the roles of disease and climate change in amphibian declines. PLoS Biol 6: pp. 441-454 dx.doi.org/10.1371/journal.pbio.0060072" target="_blank" title="It opens in new window">CrossRef
2. Roelants, K, Gower, DJ, Wilkinson, M, Loader, SP, Biju, SD, Guillaume, K, Moriau, L, Bossuyt, F (2007) Global patterns of diversification in the history of modern amphibians. Proc Natl Acad Sci U S A 104: pp. 887-892 dx.doi.org/10.1073/pnas.0608378104" target="_blank" title="It opens in new window">CrossRef
3. Blaustein, AR, Bancroft, BA (2007) Amphibian population declines: Evolutionary considerations. Bioscience 57: pp. 437-444 dx.doi.org/10.1641/B570517" target="_blank" title="It opens in new window">CrossRef
4. Carey, C, Cohen, N, Rollins-Smith, L (1999) Amphibian declines: an immunological perspective. Dev Comp Immunol 23: pp. 459-472 dx.doi.org/10.1016/S0145-305X(99)00028-2" target="_blank" title="It opens in new window">CrossRef
5. Hayes, TB, Falso, P, Gallipeau, S, Stice, M (2010) The cause of global amphibian declines: a developmental endocrinologist鈥檚 perspective. J Exp Biol 213: pp. 921-933 dx.doi.org/10.1242/jeb.040865" target="_blank" title="It opens in new window">CrossRef
6. Bank, MS, Crocker, JB, Davis, S, Brotherton, DK, Cook, R, Behler, J, Connery, B (2006) Population decline of northern dusky salamanders at Acadia National Park, Maine, USA. Biol Conserv 130: pp. 230-238 dx.doi.org/10.1016/j.biocon.2005.12.033" target="_blank" title="It opens in new window">CrossRef
7. Fellers, GM, McConnell, LL, Pratt, D, Datta, S (2004) Pesticides in mountain yellow-legged frogs (Rana muscosa) from the Sierra Nevada Mountains of California, USA. Environ Toxicol Chem 23: pp. 2170-2177 dx.doi.org/10.1897/03-491" target="_blank" title="It opens in new window">CrossRef
8. Reeder, AL, Ruiz, MO, Pessier, A, Brown, LE, Levengood, JM, Phillips, CA, Wheeler, MB, Warner, RE, Beasley, VR (2005) Intersexuality and the cricket frog decline: Historic and geographic trends. Environ Health Perspect 113: pp. 261-265 dx.doi.org/10.1289/ehp.7276" target="_blank" title="It opens in new window">CrossRef
9. Sparling, DW, Fellers, GM (2009) Toxicity of two insecticides to California, USA, anurans and its relevance to declining amphibian populations. Environ Toxicol Chem 28: pp. 1696-1703 dx.doi.org/10.1897/08-336.1" target="_blank" title="It opens in new window">CrossRef
10. Fedorenkova, A, Vonk, JA, Lenders, HJR, Creemers, RCM, Breure, AM, Hendriks, AJ (2012) Ranking ecological risks of multiple chemical stressors on amphibians. Environ Toxicol Chem 31: pp. 1416-1421 dx.doi.org/10.1002/etc.1831" target="_blank" title="It opens in new window">CrossRef
11. Kaplan, M (2009) Amphibians rarely give earliest warning of pollution. Nature.
12. Fernandez, M, L鈥橦aridon, J (1992) Influence of lighting conditions on toxicity and genotoxicity of various PAH in the newt in vivo. Mutat Res 298: pp. 31-41 dx.doi.org/10.1016/0165-1218(92)90026-V" target="_blank" title="It opens in new window">CrossRef
13. Hatch, AC, Burton, GA (1998) Effects of photoinduced toxicity of fluoranthene on amphibian embryos and larvae. Environ Toxicol Chem 17: pp. 1777-1785 dx.doi.org/10.1002/etc.5620170918" target="_blank" title="It opens in new window">CrossRef
14. Matson, CW, Gillespie, AM, McCarthy, C, McDonald, TJ, Bickham, JW, Sullivan, R, Donnelly, KC (2009) Wildlife toxicology: biomarkers of genotoxic exposures at a hazardous waste site. Ecotoxicology 18: pp. 886-898 dx.doi.org/10.1007/s10646-009-0350-1" target="_blank" title="It opens in new window">CrossRef
15. Mouchet, F, Gauthier, L, Mailhes, C, Ferrier, V, Devaux, A (2005) Comparative study of the comet assay and the micronucleus test in amphibian larvae (Xenopus laevis) using benzo(a)pyrene, ethyl methanesulfonate, and methyl methanesulfonate: Establishment of a positive control in the amphibian comet assay. Environ Toxicol 20: pp. 74-84 dx.doi.org/10.1002/tox.20080" target="_blank" title="It opens in new window">CrossRef
16. Reynaud, S, Worms, IAM, Veyrenc, S, Portier, J, Maitre, A, Miaud, C, Raveton, M (2012) Toxicokinetic of benzo a pyrene and fipronil in female green frogs (Pelophylax kl. esculentus). Environ Pollut 161: pp. 206-214 dx.doi.org/10.1016/j.envpol.2011.10.029" target="_blank" title="It opens in new window">CrossRef
17. Stabenau, EK, Giczewski, DT, Maillacheruvu, KY (2006) Uptake and elimination of naphthalene from liver, lung, and muscle tissue in the leopard frog (Rana pipiens). J Environ Sci Health A Tox Hazard Subst Environ Eng 41: pp. 1449-1461 dx.doi.org/10.1080/10934520600754029" target="_blank" title="It opens in new window">CrossRef
18. Ueda, H, Ikenaka, Y, Nakayama, SM, Tanaka-Ueno, T, Ishizuka, M (2011) Phase-II conjugation ability for PAH metabolism in amphibians: characteristics and inter-species differences. Aquat Toxicol 105: pp. 337-343 dx.doi.org/10.1016/j.aquatox.2011.07.004" target="_blank" title="It opens in new window">CrossRef
19. Di Croce, L, Bruscalupi, G, Trentalance, A (1997) Independent responsiveness of frog liver low-density lipoprotein receptor and HMGCoA reductase to estrogen treatment. Pflugers Arch 435: pp. 107-111 dx.doi.org/10.1007/s004240050489" target="_blank" title="It opens in new window">CrossRef
20. Padros, J, Pelletier, E, Ribeiro, CO (2003) Metabolic interactions between low doses of benzo[a]pyrene and tributyltin in arctic charr (Salvelinus alpinus): a long-term in vivo study. Toxicol Appl Pharmacol 192: pp. 45-55 dx.doi.org/10.1016/S0041-008X(02)00042-X" target="_blank" title="It opens in new window">CrossRef
21. Willett, KL, Gardinali, PR, Lienesch, LA, Di Giulio, RT (2000) Comparative metabolism and excretion of benzo(a)pyrene in 2 species of ictalurid catfish. Toxicol Sci 58: pp. 68-76 dx.doi.org/10.1093/toxsci/58.1.68" target="_blank" title="It opens in new window">CrossRef
22. Kohle, C, Bock, KW (2007) Coordinate regulation of Phase I and II xenobiotic metabolisms by the Ah receptor and Nrf2. Biochem Pharmacol 73: pp. 1853-1862 dx.doi.org/10.1016/j.bcp.2007.01.009" target="_blank" title="It opens in new window">CrossRef
23. Ma, Q (2001) Induction of CYP1A1. The AhR/DRE paradigm: transcription, receptor regulation, and expanding biological roles. Curr Drug Metab 2: pp. 149-164 dx.doi.org/10.2174/1389200013338603" target="_blank" title="It opens in new window">CrossRef
24. Nebert, DW, Dalton, TP, Okey, AB, Gonzalez, FJ (2004) Role of aryl hydrocarbon receptor-mediated induction of the CYP1 enzymes in environmental toxicity and cancer. J Biol Chem 279: pp. 23847-23850 dx.doi.org/10.1074/jbc.R400004200" target="_blank" title="It opens in new window">CrossRef
25. Penning, TM, Burczynski, ME, Hung, CF, McCoull, KD, Palackal, NT, Tsuruda, LS (1999) Dihydrodiol dehydrogenases and polycyclic aromatic hydrocarbon activation: generation of reactive and redox active o-quinones. Chem Res Toxicol 12: pp. 1-18 dx.doi.org/10.1021/tx980143n" target="_blank" title="It opens in new window">CrossRef
26. Sturgill, MG, Lambert, GH (1997) Xenobiotic-induced hepatotoxicity: mechanisms of liver injury and methods of monitoring hepatic function. Clin Chem 43: pp. 1512-1526
27. Brandt, CA, Becker, JM, Porta, A (2002) Distribution of polycyclic aromatic hydrocarbons in soils and terrestrial biota after a spill of crude oil in Trecate, Italy. Environ Toxicol Chem 21: pp. 1638-1643 dx.doi.org/10.1002/etc.5620210814" target="_blank" title="It opens in new window">CrossRef
28. Devonshire, AS, Sanders, R, Wilkes, TM, Taylor, MS, Foy, CA, Huggett, JF (2013) Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis. Methods 59: pp. 89-100 dx.doi.org/10.1016/j.ymeth.2012.07.021" target="_blank" title="It opens in new window">CrossRef
29. Gohin, M, Bobe, J, Chesnel, F (2010) Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates. BMC Genomics 11: pp. 18 dx.doi.org/10.1186/1471-2164-11-18" target="_blank" title="It opens in new window">CrossRef
30. Rosenblum, EB, Poorten, TJ, Settles, M, Murdoch, GK, Robert, J, Maddox, N, Eisen, MB (2009) Genome-wide transcriptional response of Silurana (Xenopus) tropicalis to infection with the deadly chytrid fungus. PLoS One 4: pp. e6494 dx.doi.org/10.1371/journal.pone.0006494" target="_blank" title="It opens in new window">CrossRef
31. Yang, W, Qi, Y, Bi, K, Fu, J (2012) Toward understanding the genetic basis of adaptation to high-elevation life in poikilothermic species: a comparative transcriptomic analysis of two ranid frogs, Rana chensinensis and R. kukunoris. BMC Genomics 13: pp. 588 dx.doi.org/10.1186/1471-2164-13-588" target="_blank" title="It opens in new window">CrossRef
32. van Delft, JH, Mathijs, K, Staal, YC, van Herwijnen, MH, Brauers, KJ, Boorsma, A, Kleinjans, JC (2010) Time series analysis of benzo[A]pyrene-induced transcriptome changes suggests that a network of transcription factors regulates the effects on functional gene sets. Toxicol Sci 117: pp. 381-392 dx.doi.org/10.1093/toxsci/kfq214" target="_blank" title="It opens in new window">CrossRef
33. Hellsten, U, Harland, RM, Gilchrist, MJ, Hendrix, D, Jurka, J, Kapitonov, V, Ovcharenko, I, Putnam, NH, Shu, SQ, Taher, L, Blitz, IL, Blumberg, B, Dichmann, DS, Dubchak, I, Amaya, E, Detter, JC, Fletcher, R, Gerhard, DS, Goodstein, D, Graves, T, Grigoriev, IV, Grimwood, J, Kawashima, T, Lindquist, E, Lucas, SM, Mead, PE, Mitros, T, Ogino, H, Ohta, Y, Poliakov, AV (2010) The Genome of the Western Clawed Frog Xenopus tropicalis. Science 328: pp. 633-636 dx.doi.org/10.1126/science.1183670" target="_blank" title="It opens in new window">CrossRef
34. Bowes, JB, Snyder, KA, Segerdell, E, Gibb, R, Jarabek, C, Noumen, E, Pollet, N, Vize, PD (2008) Xenbase: a Xenopus biology and genomics resource. Nucleic Acids Res 36: pp. D761-D767
35. Safholm, M, Norder, A, Fick, J, Berg, C (2012) Disrupted oogenesis in the frog Xenopus tropicalis after exposure to environmental progestin concentrations. Biol Reprod 86: pp. 126 dx.doi.org/10.1095/biolreprod.111.097378" target="_blank" title="It opens in new window">CrossRef
36. Schwendiman, AL, Propper, CR (2012) A common environmental contaminant affects sexual behavior in the clawed frog, Xenopus tropicalis. Physiol Behav 106: pp. 520-526 dx.doi.org/10.1016/j.physbeh.2012.03.035" target="_blank" title="It opens in new window">CrossRef
37. Matson, CW, Palatnikov, GM, McDonald, TJ, Autenrieth, RL, Donnelly, KC, Anderson, TA, Canas, JE, Islamzadeh, A, Bickham, JW (2005) Patterns of genotoxicity and contaminant exposure: evidence of genomic instability in the marsh frogs (Rana ridibunda) of Sumgayit, Azerbaijan. Environ Toxicol Chem 24: pp. 2055-2064 dx.doi.org/10.1897/04-250R1.1" target="_blank" title="It opens in new window">CrossRef
38. Wang, L, Camus, AC, Dong, W, Thornton, C, Willett, KL (2010) Expression of CYP1C1 and CYP1A in Fundulus heteroclitus during PAH-induced carcinogenesis. Aquat Toxicol 99: pp. 439-447 dx.doi.org/10.1016/j.aquatox.2010.06.002" target="_blank" title="It opens in new window">CrossRef
39. Bechmann, LP, Hannivoort, RA, Gerken, G, Hotamisligil, GS, Trauner, M, Canbay, A (2012) The interaction of hepatic lipid and glucose metabolism in liver diseases. J Hepatol 56: pp. 952-964 dx.doi.org/10.1016/j.jhep.2011.08.025" target="_blank" title="It opens in new window">CrossRef
40. Im, SS, Kang, SY, Kim, SY, Kim, HI, Kim, JW, Kim, KS, Ahn, YH (2005) Glucose-stimulated upregulation of GLUT2 gene is mediated by sterol response element-binding protein-1c in the hepatocytes. Diabetes 54: pp. 1684-1691 dx.doi.org/10.2337/diabetes.54.6.1684" target="_blank" title="It opens in new window">CrossRef
41. Brismar, K, Fernqvist-Forbes, E, Wahren, J, Hall, K (1994) Effect of insulin on the hepatic production of insulin-like growth factor-binding protein-1 (IGFBP-1), IGFBP-3, and IGF-I in insulin-dependent diabetes. J Clin Endocrinol Metab 79: pp. 872-878
42. Ung, CY, Lam, SH, Hlaing, MM, Winata, CL, Korzh, S, Mathavan, S, Gong, Z (2010) Mercury-induced hepatotoxicity in zebrafish: in vivo mechanistic insights from transcriptome analysis, phenotype anchoring and targeted gene expression validation. BMC Genomics 11: pp. 212 dx.doi.org/10.1186/1471-2164-11-212" target="_blank" title="It opens in new window">CrossRef
43. Wills, LP, Jung, D, Koehrn, K, Zhu, S, Willett, KL, Hinton, DE, Di Giulio, RT (2010) Comparative chronic liver toxicity of benzo[a]pyrene in two populations of the atlantic killifish (Fundulus heteroclitus) with different exposure histories. Environ Health Perspect 118: pp. 1376-1381 dx.doi.org/10.1289/ehp.0901799" target="_blank" title="It opens in new window">CrossRef
44. Aroor, AR, James, TT, Jackson, DE, Shukla, SD (2010) Differential changes in MAP kinases, histone modifications, and liver injury in rats acutely treated with ethanol. Alcohol Clin Exp Res 34: pp. 1543-1551 dx.doi.org/10.1111/j.1530-0277.2010.01239.x" target="_blank" title="It opens in new window">CrossRef
45. Yin, HQ, Kim, M, Kim, JH, Kong, G, Kang, KS, Kim, HL, Yoon, BI, Lee, MO, Lee, BH (2007) Differential gene expression and lipid metabolism in fatty liver induced by acute ethanol treatment in mice. Toxicol Appl Pharmacol 223: pp. 225-233 dx.doi.org/10.1016/j.taap.2007.06.018" target="_blank" title="It opens in new window">CrossRef
46. Frangioni, G, Atzori, A, Balzi, M, Fuzzi, G, Ghinassi, A, Pescosolido, N, Bianchi, S, Borgioli, G (2006) Thyroid and hypoxic stress in the newt Triturus carnifex. J Exp Zool A Comp Exp Biol 305: pp. 225-232 dx.doi.org/10.1002/jez.a.268" target="_blank" title="It opens in new window">CrossRef
47. Naik, A, Kosir, R, Rozman, D (2013) Genomic aspects of NAFLD pathogenesis. Genomics 102: pp. 84-95 dx.doi.org/10.1016/j.ygeno.2013.03.007" target="_blank" title="It opens in new window">CrossRef
48. Musso, G, Gambino, R, Cassader, M (2013) Cholesterol metabolism and the pathogenesis of non-alcoholic steatohepatitis. Prog Lipid Res 52: pp. 175-191 dx.doi.org/10.1016/j.plipres.2012.11.002" target="_blank" title="It opens in new window">CrossRef
49. Mudipalli, A (2007) Lead hepatotoxicity & potential health effects. Indian J Med Res 126: pp. 518-527
50. Sokolovic, M, Sokolovic, A, van Roomen, CP, Gruber, A, Ottenhoff, R, Scheij, S, Hakvoort, TB, Lamers, WH, Groen, AK (2010) Unexpected effects of fasting on murine lipid homeostasis鈥搕ranscriptomic and lipid profiling. J Hepatol 52: pp. 737-744 dx.doi.org/10.1016/j.jhep.2009.11.028" target="_blank" title="It opens in new window">CrossRef
51. Barni, S, Vaccarone, R, Bertone, V, Fraschini, A, Bernini, F, Fenoglio, C (2002) Mechanisms of changes to the liver pigmentary component during the annual cycle (activity and hibernation) of Rana esculenta L. J Anat 200: pp. 185-194 dx.doi.org/10.1046/j.0021-8782.2001.00011.x" target="_blank" title="It opens in new window">CrossRef
52. Jiang, PH, Motoo, Y, Garcia, S, Iovanna, JL, Pebusque, MJ, Sawabu, N (2006) Down-expression of tumor protein p53-induced nuclear protein 1 in human gastric cancer. World J Gastroenterol 12: pp. 691-696
53. Zhuo, L, Gong, J, Yang, R, Sheng, Y, Zhou, L, Kong, X, Cao, K (2009) Inhibition of proliferation and differentiation and promotion of apoptosis by cyclin L2 in mouse embryonic carcinoma P19 cells. Biochem Biophys Res Commun 390: pp. 451-457 dx.doi.org/10.1016/j.bbrc.2009.09.089" target="_blank" title="It opens in new window">CrossRef
54. Firrincieli, D, Zuniga, S, Rey, C, Wendum, D, Lasnier, E, Rainteau, D, Braescu, T, Falguieres, T, Boissan, M, Cadoret, A, Housset, C, Chignard, N (2013) Vitamin D nuclear receptor deficiency promotes cholestatic liver injury by disruption of biliary epithelial cell junctions in mice. Hepatology 58: pp. 1401-1412 dx.doi.org/10.1002/hep.26453" target="_blank" title="It opens in new window">CrossRef
55. Ribback, S, Calvisi, DF, Cigliano, A, Sailer, V, Peters, M, Rausch, J, Heidecke, CD, Birth, M, Dombrowski, F (2013) Molecular and metabolic changes in human liver clear cell foci resemble the alterations occurring in rat hepatocarcinogenesis. J Hepatol 58: pp. 1147-1156 dx.doi.org/10.1016/j.jhep.2013.01.013" target="_blank" title="It opens in new window">CrossRef
56. Seki, E, Brenner, DA, Karin, M (2012) A liver full of JNK: signaling in regulation of cell function and disease pathogenesis, and clinical approaches. Gastroenterology 143: pp. 307-320 dx.doi.org/10.1053/j.gastro.2012.06.004" target="_blank" title="It opens in new window">CrossRef
57. Villa, A, Della Torre, S, Stell, A, Cook, J, Brown, M, Maggi, A (2012) Tetradian oscillation of estrogen receptor alpha is necessary to prevent liver lipid deposition. Proc Natl Acad Sci U S A 109: pp. 11806-11811 dx.doi.org/10.1073/pnas.1205797109" target="_blank" title="It opens in new window">CrossRef
58. Swedenborg, E, Pongratz, I (2010) AhR and ARNT modulate ER signaling. Toxicology 268: pp. 132-138 dx.doi.org/10.1016/j.tox.2009.09.007" target="_blank" title="It opens in new window">CrossRef
59. Bussmann, UA, Perez Saez, JM, Bussmann, LE, Baranao, JL (2013) Aryl hydrocarbon receptor activation leads to impairment of estrogen-driven chicken vitellogenin promoter activity in LMH cells. Comp Biochem Physiol C Toxicol Pharmacol 157: pp. 111-118 dx.doi.org/10.1016/j.cbpc.2012.10.006" target="_blank" title="It opens in new window">CrossRef
60. Bugel, SM, White, LA, Cooper, KR (2013) Inhibition of vitellogenin gene induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin is mediated by aryl hydrocarbon receptor 2 (AHR2) in zebrafish (Danio rerio). Aquat Toxicol 126: pp. 1-8 dx.doi.org/10.1016/j.aquatox.2012.09.019" target="_blank" title="It opens in new window">CrossRef
61. Hazlehurst, JM, Tomlinson, JW (2013) Non-alcoholic fatty liver disease in common endocrine disorders. Eur J Endocrinol 169: pp. R27-R37 dx.doi.org/10.1530/EJE-13-0296" target="_blank" title="It opens in new window">CrossRef
62. Chen, ZS, Guo, Y, Belinsky, MG, Kotova, E, Kruh, GD (2005) Transport of bile acids, sulfated steroids, estradiol 17-beta-D-glucuronide, and leukotriene C4 by human multidrug resistance protein 8 (ABCC11). Mol Pharmacol 67: pp. 545-557 dx.doi.org/10.1124/mol.104.007138" target="_blank" title="It opens in new window">CrossRef
63. Sneitz, N, Krishnan, K, Covey, DF, Finel, M (2011) Glucuronidation of the steroid enantiomers ent-17beta-estradiol, ent-androsterone and ent-etiocholanolone by the human UDP-glucuronosyltransferases. J Steroid Biochem Mol Biol 127: pp. 282-288 dx.doi.org/10.1016/j.jsbmb.2011.08.008" target="_blank" title="It opens in new window">CrossRef
64. Fernandez, C, Lindholm, M, Krogh, M, Lucas, S, Larsson, S, Osmark, P, Berger, K, Boren, J, Fielding, B, Frayn, K, Holm, C (2008) Disturbed cholesterol homeostasis in hormone-sensitive lipase-null mice. Am J Physiol Endocrinol Metab 295: pp. E820-E831 dx.doi.org/10.1152/ajpendo.90206.2008" target="_blank" title="It opens in new window">CrossRef
65. Dortch, MS, Zakikhani, M, Kim, SC, Steevens, JA (2008) Modeling water and sediment contamination of Lake Pontchartrain following pump-out of Hurricane Katrina floodwater. J Environ Manage 87: pp. 429-442
66. Trapnell, C, Pachter, L, Salzberg, SL (2009) TopHat: discovering splice junctions with RNA-Seq. Bioinformatics 25: pp. 1105-1111 dx.doi.org/10.1093/bioinformatics/btp120" target="_blank" title="It opens in new window">CrossRef
67. Anders, S, Huber, W (2010) Differential expression analysis for sequence count data. Genome Biol 11: pp. R106 dx.doi.org/10.1186/gb-2010-11-10-r106" target="_blank" title="It opens in new window">CrossRef
68. Saeed, AI, Sharov, V, White, J, Li, J, Liang, W, Bhagabati, N, Braisted, J, Klapa, M, Currier, T, Thiagarajan, M, Sturn, A, Snuffin, M, Rezantsev, A, Popov, D, Ryltsov, A, Kostukovich, E, Borisovsky, I, Liu, Z, Vinsavich, A, Trush, V, Quackenbush, J (2003) TM4: a free, open-source system for microarray data management and analysis. Biotechniques 34: pp. 374-378
69. D鈥橦aeseleer, P (2005) How does gene expression clustering work?. Nat Biotechnol 23: pp. 1499-1501 dx.doi.org/10.1038/nbt1205-1499" target="_blank" title="It opens in new window">CrossRef
70. Huang, DW, Sherman, BT, Lempicki, RA (2009) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 4: pp. 44-57 dx.doi.org/10.1038/nprot.2008.211" target="_blank" title="It opens in new window">CrossRef
71. Huang, DW, Sherman, BT, Lempicki, RA (2009) Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res 37: pp. 1-13 dx.doi.org/10.1093/nar/gkn923" target="_blank" title="It opens in new window">CrossRef
72. Zheng, D, Kille, P, Feeney, GP, Cunningham, P, Handy, RD, Hogstrand, C (2010) Dynamic transcriptomic profiles of zebrafish gills in response to zinc depletion. BMC Genomics 11: pp. 548 dx.doi.org/10.1186/1471-2164-11-548" target="_blank" title="It opens in new window">CrossRef
73. Zheng, DL, Kille, P, Feeney, GP, Cunningham, P, Handy, RD, Hogstrand, C (2010) Dynamic transcriptomic profiles of zebrafish gills in response to zinc supplementation. BMC Genomics 11: pp. 553 dx.doi.org/10.1186/1471-2164-11-553" target="_blank" title="It opens in new window">CrossRef
74. Pfaffl, MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: pp. 2002-2007 dx.doi.org/10.1093/nar/29.9.e45" target="_blank" title="It opens in new window">CrossRef
75. Beyer, J, Jonsson, G, Porte, C, Krahn, MM, Ariese, F (2010) Analytical methods for determining metabolites of polycyclic aromatic hydrocarbon (PAH) pollutants in fish bile: A review. Environ Toxicol Pharmacol 30: pp. 224-244 dx.doi.org/10.1016/j.etap.2010.08.004" target="_blank" title="It opens in new window">CrossRef
76. Aas, E, Baussant, T, Balk, L, Liewenborg, B, Andersen, OK (2000) PAH metabolites in bile, cytochrome P4501A and DNA adducts as environmental risk parameters for chronic oil exposure: a laboratory experiment with Atlantic cod. Aquat Toxicol 51: pp. 241-258 dx.doi.org/10.1016/S0166-445X(00)00108-9" target="_blank" title="It opens in new window">CrossRef - 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
- 出版者：BioMed Central
- ISSN：1471-2164
文摘
Background Despite numerous studies suggesting that amphibians are highly sensitive to cumulative anthropogenic stresses, the role pollutants play in the decline of amphibian populations remains unclear. Amongst the most common aquatic contaminants, polycyclic aromatic hydrocarbons (PAHs) have been shown to induce several adverse effects on amphibian species in the larval stages. Conversely, adults exposed to high concentrations of the ubiquitous PAH, benzo[a]pyrene (BaP), tolerate the compound thanks to their highly efficient hepatic detoxification mechanisms. Due to this apparent lack of toxic effect on adults, no studies have examined in depth the potential toxicological impact of PAH on the physiology of adult amphibian livers. This study sheds light on the hepatic responses of Xenopus tropicalis when exposed to high environmentally relevant concentrations of BaP, by combining a high throughput transcriptomic approach (mRNA deep sequencing) and a characterization of cellular and physiological modifications to the amphibian liver. Results Transcriptomic changes observed in BaP-exposed Xenopus were further characterized using a time-dependent enrichment analysis, which revealed the pollutant-dependent gene regulation of important biochemical pathways, such as cholesterol biosynthesis, insulin signaling, adipocytokines signaling, glycolysis/gluconeogenesis and MAPK signaling. These results were substantiated at the physiological level with the detection of a pronounced metabolic disorder resulting in a possible insulin resistance-like syndrome phenotype. Hepatotoxicity induced by lipid and cholesterol metabolism impairments was clearly identified in BaP-exposed individuals. Conclusions Our data suggested that BaP may disrupt overall liver physiology, and carbohydrate and cholesterol metabolism in particular, even after short-term exposure. These results are further discussed in terms of how this deregulation of liver physiology can lead to general metabolic impairment in amphibians chronically exposed to contaminants, thereby illustrating the role xenobiotics might play in the global decline in amphibian populations.