The characterization and purification of a human transcription factor modulating the glutathione peroxidase gene in response to oxygen tension
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- 作者：Frank Merante ; Svetlana M. Altamentova ; Donald A.G. Mickle ; Richard D. Weisel ; Bradley J. Thatcher ; Brian M. Martin ; John G. Marshall ; Laura C. Tumiati ; Douglas B. Cowan and Ren-Ke Li
- 关键词：DNA binding proteins ; glutathione peroxidase ; hypoxia ; Ku ; antigen ; protein purification ; transcription factors
- 刊名：Molecular and Cellular Biochemistry
- 出版年：2002
- 出版时间：2002
- 年：2002
- 卷：229
- 期：1/2
- 页码：73-83
- 全文大小：164 KB
文摘
An oxygen responsive transcription factor regulating human glutathione peroxidase gene (GPx) through two oxygen responsive elements (ORE1 and ORE2) has been purified and characterized by sequence-specific DNA affinity chromatography. The DNA binding activity, termed Oxygen Responsive Element Binding Protein (OREBP), was partially represented by a 77 kD polypeptide (p70) possessing a blocked N-terminus. The p70 subunit co-eluted with an 86 kD subunit (p80) from affinity columns. N-terminal sequencing analysis of the 86 kD component revealed that this protein represented the larger member of the Ku antigen complex. The identity of the purified 77 kD subunit was determined by Western blot analysis using an antibody directed against the p70 protein. In addition to binding the GPx-ORE, the OREBP was itself regulated by oxygen tension. It was found that the abundance of the ORE binding activity was decreased in cells maintained at low oxygen tension (40 mm Hg). Anti-Ku-antibodies specifically supershifted the OREBP-ORE DNA complex. These observations further add to the numerous nuclear roles of the Ku-transcription factor.