Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors
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- 作者：Mohammad Sadra Shirazi (1)
Banafsheh Heidari (1)
Abolfazl Shirazi (1) (2)
Amir Hassan Zarnani (3)
Mahmood Jeddi-Tehrani (4)
Maryam Rahmati-Ahmadabadi (1)
Mohammad Mehdi Naderi (1)
Bahareh Behzadi (1)
Moretza Farab (1)
Ali Sarvari (1)
Sara Borjian-Boroujeni (1)
Mohammad Mehdi Akhondi (1) - 关键词：PGP9.5 ; KIT ; Goat ; Spermatogonial stem cell ; Growth factors ; Mouse
- 刊名：Journal of Assisted Reproduction and Genetics
- 出版年：2014
- 出版时间：November 2014
- 年：2014
- 卷：31
- 期：11
- 页码：1519-1531
- 全文大小：2,298 KB
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18. Kanatsu-Shinohara M, Miki H, Inoue K, Ogonuki N, Toyokuni S, Ogura A, et al. Long-term culture of mouse male germline stem cells under serum- or feeder-free conditions. Biol Reprod. 2005;72:985-1. Sadra Shirazi (1)
Banafsheh Heidari (1)
Abolfazl Shirazi (1) (2)
Amir Hassan Zarnani (3)
Mahmood Jeddi-Tehrani (4)
Maryam Rahmati-Ahmadabadi (1)
Mohammad Mehdi Naderi (1)
Bahareh Behzadi (1)
Moretza Farab (1)
Ali Sarvari (1)
Sara Borjian-Boroujeni (1)
Mohammad Mehdi Akhondi (1)
1. Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
2. Research Institute of Animal Embryo Technology, Shahrekord University, P.O. Box: 19615-177, 1936773493, Shahrekord, Iran
3. Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
4. Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran - ISSN：1573-7330
文摘
Purpose The present study by using different growth factors was aimed to develop the best practical culture condition for purification of goat undifferentiated SSCs and their colonization under in vitro and in vivo conditions. Methods The enzymatically isolated SSCs obtained from one month old goat testes were cultured in DMEM plus FCS supplemented with different sets of growth factors (GDNF, LIF, bFGF, and EGF) for 2?weeks. At the end of each week, the morphological characteristics of cells and colonies alongside with purification rate of undifferentiated type A spermatogonia were evaluated by immunocytochemical staining and flow cytometry. Results The number and size of colonies in treatment groups were significantly (P--.01) higher than corresponding values in control group. In immunocytochemical evaluation, the proportion of KIT and PGP9.5 positive cells were significantly (P--.001) higher in control and treatment groups, respectively. Conclusions The culture medium comprising all four growth factors, especially the one supplemented with the higher concentration of GDNF, was superior to the other groups with respect to the population of undifferentiated type A spermatogonia and its propagation in culture system. Additionally, goat SSCs could colonize within the mouse testis following xenotransplantation.