Histological and SEM analysis of root cementum following irradiation with Er:YAG and CO2 lasers
详细信息 查看全文
- 作者：Aslam Almehdi (1) (2)
Akira Aoki (1)
Shizuko Ichinose (3)
Yoichi Taniguchi (1)
Katia M. Sasaki (4)
Kenichiro Ejiri (1)
Masanori Sawabe (1)
Chanthoeun Chui (1) (2)
Sayaka Katagiri (1)
Yuichi Izumi (1) (2) - 关键词：Cementum ; Alteration ; Microstructure ; Er ; YAG laser ; CO2 laser ; Ultrasonic scaler
- 刊名：Lasers in Medical Science
- 出版年：2013
- 出版时间：January 2013
- 年：2013
- 卷：28
- 期：1
- 页码：203-213
- 全文大小：1013KB
- 参考文献：1. Socransky SS, Haffajee AD (1992) The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol 63:322-31 CrossRef
2. Kinane DF (2001) Causation and pathogenesis of periodontal disease. Periodontol 2000 25:8-0 CrossRef
3. Cobb CM (2002) Clinical significance of non-surgical periodontal therapy: an evidence-based perspective of scaling and root planing. J Clin Periodontol 29(Suppl 2):6-6
4. Oda S, Nitta H, Setoguchi T, Izumi Y, Ishikawa I (2004) Current concepts and advances in manual and power-driven instrumentation. Periodontol 2000 36:45-8 CrossRef
5. Adriaens PA, Edwards CA, De Boever JA, Loesche WJ (1988) Ultrastructural observations on bacterial invasion in cementum and radicular dentin of periodontally diseased human teeth. J Periodontol 59:493-03 CrossRef
6. Aoki A, Sasaki K, Watanabe H, Ishikawa I (2004) Lasers in non-surgical periodontal therapy. Periodontol 2000 36:59-7 CrossRef
7. Ishikawa I, Aoki A, Takasaki AA, Mizutani K, Sasaki KM, Izumi Y (2009) Application of lasers in periodontics: true innovation or myth? Periodontol 2000 50:90-26 CrossRef
8. Pick RM, Colvard MD (1993) Current status of lasers in soft tissue dental surgery. J Periodontol 64:589-02 CrossRef
9. Hibst R, Keller U (1989) Experimental studies of the application of the Er:YAG laser on dental hard substances: I. Measurement of the ablation rate. Lasers Surg Med 9:338-44 CrossRef
10. Keller U, Hibst R (1989) Experimental studies of the application of the Er:YAG laser on dental hard substances: II. Light microscopic and SEM investigations. Lasers Surg Med 9:345-51 CrossRef
11. Kayano T, Ochiai S, Kiyono K, Yamamoto H, Nakajima S, Mochizuki T (1991) Effect of Er:YAG laser irradiation on human extracted teeth. J Clin Laser Med Surg 9:147-50
12. Hale GM, Querry MR (1973) Optical constants of water in the 200-nm to 200-μm wavelength region. Appl Opt 12:555-63 CrossRef
13. Watanabe H, Ishikawa I, Suzuki M, Hasegawa K (1996) Clinical assessments of the erbium:YAG laser for soft tissue surgery and scaling. J Clin Laser Med Surg 14:67-5
14. Schwarz F, Sculean A, Georg T, Reich E (2001) Periodontal treatment with an Er:YAG laser compared to scaling and root planing. A controlled clinical study. J Periodontol 72:361-67 CrossRef
15. Aoki A, Ando Y, Watanabe H, Ishikawa I (1994) In vitro studies on laser scaling of subgingival calculus with an erbium:YAG laser. J Periodontol 65:1097-106 CrossRef
16. Aoki A, Miura M, Akiyama F, Nakagawa N, Tanaka J, Oda S, Watanabe H, Ishikawa I (2000) In vitro evaluation of Er:YAG laser scaling of subgingival calculus in comparison with ultrasonic scaling. J Periodontol Res 35:266-77 CrossRef
17. Schwarz F, Putz N, Georg T, Reich E (2001) Effect of an Er:YAG laser on periodontally involved root surfaces: an in vivo and in vitro SEM comparison. Lasers Surg Med 29:328-35 CrossRef
18. Schwarz F, Sculean A, Berakdar M, Szathmari L, Georg T, Becker J (2003) In vivo and in vitro effects of an Er:YAG laser, a GaAlAs diode laser, and scaling and root planing on periodontally diseased root surfaces: a comparative histologic study. Lasers Surg Med 32:359-66 CrossRef
19. Eberhard J, Ehlers H, Falk W, Acil Y, Albers HK, Jepsen S (2003) Efficacy of subgingival calculus removal with Er:YAG laser compared to mechanical debridement: an in situ study. J Clin Periodontol 30:511-18 CrossRef
20. Schwarz F, Aoki A, Becker J, Sculean A (2008) Laser application in non-surgical periodontal therapy: a systematic review. J Clin Periodontol 35:29-4 CrossRef
21. Gaspirc B, Skaleric U (2007) Clinical evaluation of periodontal surgical treatment with an Er:YAG laser: 5-year results. J Periodontol 78:1864-871 CrossRef
22. The Research, Science and Therapy Committee of the American Academy of Periodontology (2002) Lasers in periodontics (Academy report), authored by Cohen RE and Ammons WF, revised by Rossman JA. J Periodontol 73:1231-239 CrossRef
23. Aoki A, Ishikawa I, Yamada T, Otsuki M, Watanabe H, Tagami J, Ando Y, Yamamoto H (1998) Comparison between Er:YAG laser and conventional technique for root caries treatment in vitro. J Dent Res 77:1404-414 CrossRef
24. Maruyama H, Aoki A, Sasaki KM, Takasaki AA, Iwasaki K, Ichinose S, Oda S, Ishikawa I, Izumi Y (2008) The effect of chemical and/or mechanical conditioning on the Er:YAG laser-treated root cementum: analysis of surface morphology and periodontal ligament fibroblast attachment. Lasers Surg Med 40:211-22 CrossRef
25. Sasaki KM, Aoki A, Masuno H, Ichinose S, Yamada S, Ishikawa I (2002) Compositional analysis of root cementum and dentin after Er:YAG laser irradiation compared with CO2 lased and intact roots using Fourier transformed infrared spectroscopy. J Periodontol Res 37:50-9 CrossRef
26. Sasaki KM, Aoki A, Ichinose S, Ishikawa I (2002) Ultrastructural analysis of bone tissue irradiated by Er:YAG laser. Lasers Surg Med 31:322-32 CrossRef
27. Israel M, Cobb CM, Rossmann JA, Spencer P (1997) The effects of CO2, Nd:YAG and Er:YAG lasers with and without surface coolant on tooth root surfaces. An in vitro study. J Clin Periodontol 24:595-02 CrossRef
28. Sasaki KM, Aoki A, Ichinose S, Ishikawa I (2002) Morphological analysis of cementum and root dentin after Er:YAG laser irradiation. Lasers Surg Med 31:79-5 CrossRef
29. Featherstone JDB (2000) Caries detection and prevention with laser energy. Dent Clin North Am 44:955-69
30. Fried D, Zuerlein M, Featherstone JDB, Seka W, McCormack SM (1997) IR laser ablation of dental enamel: mechanistic dependence on the primary absorber. Appl Surf Sci 127:852-56 CrossRef
31. Seka W, Featherstone JDB, Fried D, Visuri SR, Walsh JT (1996) Laser ablation of dental hard tissue: from explosive ablation to plasma-mediated ablation. Proc SPIE 2672:144-58 CrossRef
32. Kuroda S, Fowler BO (1984) Compositional, structural, and phase changes in in vitro laser-irradiated human tooth enamel. Calcif Tissue Int 36:361-69 CrossRef
33. Fowler BO, Kuroda S (1986) Changes in heated and in laser-irradiated human tooth enamel and their probable effects on solubility. Calcif Tissue Int 38:197-08 CrossRef
34. Rohanizadeh R, LeGeros RZ, Fan D, Jean A, Daculsi G (1999) Ultrastructural properties of laser-irradiated and heat-treated dentin. J Dent Res 78:1829-835 CrossRef
35. Mizutani K, Aoki A, Takasaki AA, Kinoshita A, Hayashi C, Oda S, Ishikawa I (2006) Periodontal tissue healing following flap surgery using an Er:YAG laser in dogs. Lasers Surg Med 38:314-24 CrossRef
36. Schwarz F, Sculean A, Berakdar M, Georg T, Reich E, Becker J (2003) Periodontal treatment with an Er:YAG laser or scaling and root planing. A 2-year follow-up split-mouth study. J Periodontol 74:590-96 CrossRef
37. Schwarz F, Sculean A, Berakdar M, Georg T, Reich E, Becker J (2003) Clinical evaluation of an Er:YAG laser combined with scaling and root planing for non-surgical periodontal treatment. A controlled, prospective clinical study. J Clin Periodontol 30:26-4 CrossRef
38. Pourzarandian A, Watanabe H, Aoki A, Ichinose S, Sasaki K, Nitta H, Ishikawa I (2004) Histological and TEM examination of early stages of bone healing after Er:YAG laser irradiation. Photomed Laser Surg 22:355-63 CrossRef
39. Schwarz F, Aoki A, Sculean A, Georg T, Scherbaum W, Becker J (2003) In vivo effects of an Er:YAG laser, an ultrasonic system and scaling and root planing on the biocompatibility of periodontally diseased root surfaces in cultures of human PDL fibroblasts. Lasers Surg Med 33:140-47 CrossRef
40. Belal MH, Watanabe H, Ichinose S, Ishikawa I (2007) Effect of Er:YAG laser combined with rhPDGF-BB on attachment of cultured fibroblasts to periodontally involved root surfaces. J Periodontol 78:1329-341 CrossRef
41. Nicoll BK, Peters RJ (1998) Heat generation during ultrasonic instrumentation of dentin as affected by different irrigation methods. J Periodontol 69:884-88 CrossRef
42. LeGeros RZ (1975) The unit-cell dimensions of human enamel apatite: effect of chloride incorporation. Arch Oral Biol 20:63-1 CrossRef
43. Eguro T (1998) Structural and compositional changes of human enamel by the Er:YAG laser irradiation. J Jpn Conserv Dent 41:582-95 - 作者单位：Aslam Almehdi (1) (2)
Akira Aoki (1)
Shizuko Ichinose (3)
Yoichi Taniguchi (1)
Katia M. Sasaki (4)
Kenichiro Ejiri (1)
Masanori Sawabe (1)
Chanthoeun Chui (1) (2)
Sayaka Katagiri (1)
Yuichi Izumi (1) (2)
1. Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan
2. Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Tokyo, Japan
3. Research Center for Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
4. Department of Dentistry, Brasilia University, Brasillia, Brazil - ISSN：1435-604X
文摘
Recently, the Er:YAG and CO2 lasers have been applied in periodontal therapy. However, the characteristics of laser-irradiated root cementum have not been fully analyzed. The aim of this study was to precisely analyze the alterations of root cementum treated with the Er:YAG and the CO2 lasers, using non-decalcified thin histological sections. Eleven cementum plates were prepared from extracted human teeth. Pulsed Er:YAG laser contact irradiation was performed in a line at 40?mJ/pulse (14.2?J/cm2/pulse) and 25?Hz (1.0?W) under water spray. Continuous CO2 laser irradiation was performed in non-contact mode at 1.0?W, and ultrasonic instrumentation was performed as a control. The treated samples were subjected to stereomicroscopy, scanning electron microscopy (SEM), light microscopy and SEM energy dispersive X-ray spectroscopy (SEM-EDS). The Er:YAG laser-treated cementum showed minimal alteration with a whitish, slightly ablated surface, whereas CO2 laser treatment resulted in distinct carbonization. SEM analysis revealed characteristic micro-irregularities of the Er:YAG-lased surface and the melted, resolidified appearance surrounded by major and microcracks of the CO2-lased surface. Histological analysis revealed minimal thermal alteration and structural degradation of the Er:YAG laser-irradiated cementum with an affected layer of approximately 20-μm thickness, which partially consisted of two distinct affected layers. The CO2-lased cementum revealed multiple affected layers showing different structures/staining with approximately 140?μm thickness. Er:YAG laser irradiation used with water cooling resulted in minimal cementum ablation and thermal changes with a characteristic microstructure of the superficial layer. In contrast, CO2 laser irradiation produced severely affected distinct multiple layers accompanied by melting and carbonization.