Histochemical, ultrastructural, and three-dimensional observation of smooth muscle cells in human gastric mucosa
详细信息 查看全文
- 作者：Kenichi Arai ; Hiroyoshi Ota ; Eiko Hidaka ; Masayoshi Hayama ; Kenji Sano ; Atsushi Sugiyama ; Taiji Akamatsu and Tsutomu Katsuyama
- 关键词：Stomach ; Smooth muscle cell ; Myofibroblast ; Confocal laser scanning microscope
- 刊名：Histochemistry and Cell Biology
- 出版年：2004
- 出版时间：March 2004
- 年：2004
- 卷：121
- 期：3
- 页码：229-237
- 全文大小：1,004 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Anatomy
Medicine/Public Health, general - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-119X
文摘
The present study was undertaken to clarify the histochemical and ultrastructural properties and the three-dimensional distribution of the smooth muscle cells (SMCs) located in the lamina propria (LP) of the human gastric mucosa. Standard paraffin sections obtained from stomachs surgically resected for gastric cancer were immunostained for alpha-smooth muscle actin (alpha-SMA), vimentin, desmin, laminin, and type IV collagen. In addition, 100-m-thick sections were fluorostained for alpha-SMA and CD34, while three-dimensional images were prepared by confocal laser scanning microscope. Ultrastructural studies were carried out using normal gastric biopsy specimens. The results indicated that SMCs in the LP differed between the upper and lower regions, SMCs in the lower LP being fairly typical SMCs, whereas those in the upper LP had apparently lost reactivity for desmin but gained that for vimentin. The basal lamina became sparser, but a fibronexus was occasionally seen in SMCs in the upper LP. Three-dimensional images revealed bundles of SMCs in the upper LP encircling several foveolae to form acinus-like structures and, in the upper LP, SMCs branching into fine fibrils with a brush-like (corpus) or besom-like (i.e., a twiggy witchs broom) appearance (antrum).