Crossbreeding of a metallic color carnation and diversification of the peculiar coloration by ion-beam irradiation

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• 作者：Masachika Okamura (1)
  Masayoshi Nakayama (2)
  Naoyuki Umemoto (1)
  Emilio A. Cano (3)
  Yoshihiro Hase (4)
  Yuzo Nishizaki (5)
  Nobuhiro Sasaki (5)
  Yoshihiro Ozeki (5)
  
• 关键词：Carnation ; Acylation ; Glycosylation ; Ion ; beam irradiation ; Anthocyanic vacuolar inclusions ; Metallic color
• 刊名：Euphytica
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：191
• 期：1
• 页码：45-56
• 全文大小：628KB
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  5. Matsuba Y, Sasaki N, Tera M, Okamura M, Abe Y, Okamoto E, Nakamura H, Funabashi H, Takatsu M, Saito M, Matsuoka H, Nagasawa K, Ozeki Y (2010) A novel glucosylation reaction on anthocyanis catalyzed by acyl-glucose dependent glucosyltransferase in the petals of carnation and delphinium. Plant Cell 22:3374鈥?389. doi:10.1105/tpc.110.077487 CrossRef
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  10. Nishizaki Y, Matsuba Y, Okamoto E, Okamura M, Ozeki Y, Sasaki N (2011) Structure of the acyl-glucose-dependent anthocyanin 5- / O-glucosyltransferase gene in carnations and its disruption by transposable elements in some varieties. Mol Genet Genomics 286:383鈥?94. doi:10.1007/s00438-011-0655-7 CrossRef
  11. Okamura M, Yasuno N, Ohtsuka M, Tanaka A, Shikazono N, Hase Y (2003) Wide variety of flower-color and -shape mutants regenerated from leaf cultures irradiated with ion beams. Nucl Instrum Methods Phys Res B 206:574鈥?78. doi:org/10.1016/S0168-583X(03)00835-8 CrossRef
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• 作者单位：Masachika Okamura (1)
  Masayoshi Nakayama (2)
  Naoyuki Umemoto (1)
  Emilio A. Cano (3)
  Yoshihiro Hase (4)
  Yuzo Nishizaki (5)
  Nobuhiro Sasaki (5)
  Yoshihiro Ozeki (5)
  
  1. Central Laboratories for Key Technologies, Kirin Company, Ltd., Fukuura 1-13-5, Kanazawa, Yokohama, Kanagawa, 236-0004, Japan
  2. Institute of Floricultural Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8519, Japan
  3. Barberet & Blanc, S. A., Camino Viejo, 205, 30.890 Puerto Lumbreras, Murcia, Spain
  4. Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma, 370-1292, Japan
  5. Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei, Tokyo, 184-8588, Japan
  
• ISSN：1573-5060

文摘

In general, carnations (Dianthus caryophyllus) have each of four kinds of anthocyanins acylated by malic acid. A few carnation cultivars are known to display a peculiar dusky color supposedly caused by anthocyanic vacuolar inclusions (AVIs). The hereditary pattern suggests that the peculiar color is controlled by a single recessive factor tightly linked with existence of AVIs containing non-acylated anthocyanins. To diversify the peculiar color carnation, we produced a bluish purple line displaying a highly novel metallic appearance by crossbreeding. By subjecting the line to ion-beam irradiation, we generated metallic reddish purple, metallic crimson and metallic red lines. The major anthocyanin of the metallic bluish purple and reddish purple lines was pelargonidin 3,5-diglucoside, whereas that of the metallic crimson and red lines was pelargonidin 3-glucoside. All four metallic lines did not have transcripts for anthocyanin malyltransferase. Metallic crimson and red lines did not express the acyl-glucose-dependent anthocyanin 5-O-glucosyltransferase gene. In contrast to the dusky color types, metallic lines have highly condensed AVIs and water-clear vacuolar sap in the petal adaxial epidermal cells. Differences in the number of AVIs on the abaxial side were observed within mutants containing the same anthocyanin, thereby affecting their shade and hue. We demonstrated that (1) a factor generating the AVIs is inactivated anthocyanin malyltransferase gene, (2) AVIs in water-clear vacuolar sap in the adaxial epidermal cells generate the novel metallic appearance, and (3) ion beam breeding is a useful tool for increasing metallic colors by changing anthocyanin structure and the level of AVIs.