Involvement of DNase ? in apoptotic DNA fragmentation in histiocytic necrotizing lymphadenitis

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• 作者：Yoshikazu Higami ; Kazuo To ; Hiroshi Ohtani ; Kenta Masui ; Keisuke Iwasaki ; Daisuke Shiokawa ; Sei-ichi Tanuma and Isao Shimokawa
• 关键词：DNase ; Apoptosis ; Histiocytic necrotizing lymphadenitis ; Immunohistochemistry ; Terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate mediated dUTP nick end labeling
• 刊名：Virchows Archiv
• 出版年：2003
• 出版时间：August 2003
• 年：2003
• 卷：443
• 期：2
• 页码：170-174
• 全文大小：247 KB
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Pathology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-2307

文摘

DNA fragmentation induced by endonucleases is a hallmark of apoptotic process. One of the endonucleases responsible for apoptotic DNA fragmentation has been identified as DNase . It has been suggested that massive nuclear debris observed in histiocytic necrotizing lymphadenitis (HNL, Kikuchi disease) results mainly from apoptosis of T-lymphocytes rather than necrosis. To identify the role of DNase in apoptotic foci of HNL paraffin embedded tissue sections of HNL and lymphadenopathy with hyperplastic germinal centers and paracortex in the neck lymph nodes were immunohistochemically stained for DNase and the results compared with the reactivity of TdT-mediated dUTP nick end labeling (TUNEL). Immunoreactivity for DNase was detected in fragmented and condensed nuclei found abundantly in HNL as well as TUNEL reactivity. Moreover, the ratio of DNase immunoreactivity to TUNEL reactivity, which represents probability of apoptosis relevant to DNase , was higher in foci of nuclear debris in HNL than in the hyperplastic paracortex in lymphadenopathy including T-cell apoptosis. Our findings suggest that apoptosis detected in HNL depends more specifically upon DNase than apoptosis in the hyperplastic paracortex of lymphadenopathy, and that the dysregulation of DNase activation is involved in apoptosis in HNL