Development of a chloroplast DNA marker for monitoring of transgene introgression in Brassica napus L.

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• 作者：Hee-Jong Woo (1)
  Myung-Ho Lim (1)
  Kong-Sik Shin (1)
  Bianca Martins (2)
  Bum-Kyu Lee (1)
  Hyun-Suk Cho (1)
  Carol A. Mallory-Smith (2)
  
• 关键词：Brassica napus ; Chloroplast DNA marker ; cpDNA ; Intergenic region ; Simple PCR
• 刊名：Biotechnology Letters
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：35
• 期：9
• 页码：1533-1539
• 全文大小：490KB
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• 作者单位：Hee-Jong Woo (1)
  Myung-Ho Lim (1)
  Kong-Sik Shin (1)
  Bianca Martins (2)
  Bum-Kyu Lee (1)
  Hyun-Suk Cho (1)
  Carol A. Mallory-Smith (2)
  
  1. Biosafety Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, South Korea
  2. Department of Crop and Soil Science, Oregon State University, 107 Crop Science Bldg., Corvallis, OR, 97331, USA
  

文摘

Chloroplast molecular markers can provide useful information for high-resolution analysis of inter- and intra-specific variation in Brassicaceae and for differentiation between its species. Combining data generated from nuclear and chloroplast markers enables the study of seed and pollen movement, and assists in the assessment of gene-flow from genetically modified (GM) plants through hybridization studies. To develop chloroplast DNA markers for monitoring of transgene introgression in Brassica napus L., we searched for sequence variations in the chloroplast (cp) genome, and developed a simple cpDNA marker that is reliable, time-saving, and easily discriminates among 4 species (B. napus, B. rapa, Raphanus sativus, and Sinapis alba) based on PCR-product length polymorphism. This marker will be useful to identify maternal lineages and to estimate transgene movement of GM canola.