A Standardized Method for In Vivo Mouse Pancreas Imaging and Semiquantitative β Cell Mass Measurement by Dual Isotope SPECT
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- 作者：Iris Mathijs ; Catarina Xavier ; Cindy Peleman…
- 关键词：Exendin ; l ; phenylalanine ; Dual isotope SPECT ; Beta cell mass ; Pancreas imaging
- 刊名：Molecular Imaging and Biology
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：17
- 期：1
- 页码：58-66
- 全文大小：2,144 KB
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- ISSN：1860-2002
文摘
Purpose In order to evaluate future β cell tracers in vivo, we aimed to develop a standardized in vivo method allowing semiquantitative measurement of a prospective β cell tracer within the pancreas. Procedures 2-[123I]Iodo-l-phenylalanine ([123I]IPA) and [Lys40([111In]DTPA)]exendin-3 ([111In]Ex3) pancreatic uptake and biodistribution were evaluated using SPECT, autoradiography, and an ex vivo biodistribution study in a controlled unilaterally nephrectomized mouse β cell depletion model. Semiquantitative measurement of the imaging results was performed using [123I]IPA to delineate the pancreas and [111In]Ex3 as a β cell tracer. Results The uptake of [123I]IPA was highest in the pancreas. Aside from the kidneys, the uptake of [111In]Ex3 was highest in the pancreas and lungs. Autoradiography showed only uptake of [111In]Ex3 in insulin-expressing cells. Semiquantitative measurement of [111In]Ex3 in the SPECT images based on the delineation of the pancreas with [123I]IPA showed a high correlation with the [111In]Ex3 uptake data of the pancreas obtained by dissection. A strong positive correlation was observed between the relative insulin positive area and the pancreas-to-blood ratios of [111In]Ex3 uptake as determined by counting with a gamma counter and the semiquantitative analysis of the SPECT images. Conclusions [123I]IPA is a promising tracer to delineate pancreatic tissue on SPECT images. It shows a high uptake in the pancreas as compared to other abdominal tissues. This study also demonstrates the feasibility and accuracy to measure the β cell mass in vivo in an animal model of diabetes.