Screening and Research of Anti-Cancer Matrine Components Based on Hollow Fiber Cell Fishing with High-Performance Liquid Chromatography

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• 作者：Ruxin Zhang ; Shuang Hu ; Xuan Chen ; Xiaohong Bai
• 关键词：Hollow fiber liquid ; phase microextraction ; Hollow fiber cell fishing ; Cancer cell ; Receptor ; Matrine alkaloids
• 刊名：Chromatographia
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：79
• 期：3-4
• 页码：125-136
• 全文大小：3,329 KB
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• 作者单位：Ruxin Zhang (1)
  Shuang Hu (1)
  Xuan Chen (1)
  Xiaohong Bai (1)
  
  1. School of Pharmacy, College of Pharmacy, Shanxi Medical University, Taiyuan, 030001, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

Hollow fiber cell fishing with high-performance liquid chromatography (HFCF-HPLC) based on human breast cancer MCF-7 cells, human renal tubular ACHN cells, human ovarian cancer SKOV-3 cells, and hepatoma HepG-2 cells, and their cell membranes, cell organelles, or receptors was developed and employed to research the active matrine alkaloids group, such as oxymatrine, matrine, sophoridine, and oxysophocarpine, from radix sophorae flavescentis (RSF) and its injection in vitro. Hollow fiber liquid-phase microextraction with HPLC (HF-LPME-HPLC) was conducted to extract and detect the active components in vivo. Some of the structures of the active components screened were identified in vitro. The binding sites and targets of the potential active matrine compounds were preliminarily researched. The effective anti-cancer components were determined, and the efficacy material basis of both medicines on matrine alkaloids was elucidated. Before the application of HFCF-HPLC, the survival rate of cells seeded on the fiber, death rate after screening components, non-specific binding between active centers on the fiber and target components, positive and negative controls, and repeatability of the tests were investigated. Results demonstrated that HFCF-HPLC, coupled with HF-LPME-HPLC, is a simple, quick, and reliable method to screen active components, determine truly effective components, and elucidate preliminarily the characteristics of multiple components and multiple targets for traditional Chinese medicine. Keywords Hollow fiber liquid-phase microextraction Hollow fiber cell fishing Cancer cell Receptor Matrine alkaloids