Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone
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- 作者：Akihide Nampei ; Jun Hashimoto ; Kenji Hayashida ; Hideki Tsuboi ; Kenrin Shi ; Isamu Tsuji ; Hideaki Miyashita ; Takao Yamada ; Naomichi Matsukawa and Masayuki Matsumoto ; et al.
- 关键词：MEPE ; osteocyte ; immunohistochemistry ; mineralization ; human
- 刊名：Journal of Bone and Mineral Metabolism
- 出版年：2004
- 出版时间：May 2004
- 年：2004
- 卷：22
- 期：3
- 页码：176-184
- 全文大小：490 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Metabolic Diseases
Orthopedics
Internal Medicine - 出版者：Springer Japan
- ISSN：1435-5604
文摘
The matrix extracellular phosphoglycoprotein (MEPE) gene is highly expressed in tumors that cause oncogenic hypophosphatemic osteomalacia (OHO). MEPE is also known as one of the bone-tooth matrix proteins and is associated with bone mineralization. We developed a rabbit polyclonal antibody directed against recombinant human MEPE (rhMEPE) after cloning its cDNA from the cDNA library of a nasal tumor tissue causing OHO. Using this antibody, we analyzed the distribution of MEPE in human bones by immunohistochemistry. In bone specimens from normal subjects, MEPE was predominantly expressed by osteocytes and not by osteoblasts. In bone specimens from patients with osteomalacia, however, MEPE was focally expressed by deeply located osteocytes. We also compared the MEPE positivity of osteocytes in mineralized bone and non-mineralized osteoid obtained from patients with osteomalacia and osteoporosis. Among osteomalacia patients, MEPE positivity was seen in 87.5 ± 8.6% of the osteocytes from mineralized bone compared with 7.8 ± 6.4% of those from osteoid. Among osteoporosis patients, MEPE positivity was found in 95.3 ± 0.5% of the osteocytes from mineralized bone compared with 4.9 ± 5.7% of those from osteoid. MEPE was mainly expressed by osteocytes embedded in the matrix of mineralized bone from patients with osteomalacia or osteoporosis. Our data provide the first histological evidence that MEPE is predominantly expressed by osteocytes in human bone, with significant expression by osteocytes within mineralized bone.