The obligate alkaliphile Bacillus clarkii K24-1U retains extruded protons at the beginning of respiration

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• 作者：Kazuaki Yoshimune ; Hajime Morimoto ; Yu Hirano ; Junshi Sakamoto ; Hidetoshi Matsuyama and Isao Yumoto
• 关键词：Alkaliphiles ; Bacillus clarkii ; Membrane electrical potential ; Proton efflux ; Bacillus subtilis
• 刊名：Journal of Bioenergetics and Biomembranes
• 出版年：2010
• 出版时间：April, 2010
• 年：2010
• 卷：42
• 期：2
• 页码：111-116
• 全文大小：178.5 KB

文摘

Alkaliphiles grow under alkaline conditions that might be disadvantageous for the transmembrane pH gradient (ΔpH, outside acidic). In this study, the behaviors of extruded protons by the respiration of obligate alkaliphilic Bacillus clarkii K24-1U were investigated by comparison with those of neutralophilic Bacillus subtilis IAM 1026. Although whole-cell suspensions of both Bacillus species consumed oxygen immediately after the addition of air, there were lag times before the suspensions were acidified. Under alkaline conditions, the lag time for B. clarkii significantly increased, whereas that for B. subtilis decreased. In the presence of valinomycin or ETH-157, which disrupts the membrane electrical potential (Δψ), the cell suspensions of both Bacillus species acidified immediately after the addition of air. Artificial electroneutral antiporters (nigericin and monensin) that eliminate the ΔpH exhibited no significant effect on the lag times of the two Bacillus species except that monensin increased the lag times of B. clarkii. The inhibition of ATPase and the Na+ channel also exhibited little effects on the lag times. The increased lag time for B. clarkii may represent the Δψ-dependent proton retention on the outer surface of the cytoplasmic membrane to generate a sufficient ΔpH under alkaline conditions.