Application of a Competitive Internal Amplification Control for the Detection of Sapoviruses in Wastewater

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• 作者：Tanya Y. Murray (1)
  Janet Mans (1)
  Walda B. van Zyl (1)
  Maureen B. Taylor (1) (2)
  
• 关键词：Calicivirus ; Enteric virus ; Sapovirus ; Sewage ; South Africa
• 刊名：Food and Environmental Virology
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：5
• 期：1
• 页码：61-68
• 全文大小：291KB
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• 作者单位：Tanya Y. Murray (1)
  Janet Mans (1)
  Walda B. van Zyl (1)
  Maureen B. Taylor (1) (2)
  
  1. Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Private Bag X323, Arcadia, Pretoria, 0007, South Africa
  2. Tshwane Academic Division, National Health Laboratory Service, Pretoria, South Africa
  

文摘

In this study, a competitive internal amplification control (IAC) was constructed for application in the real-time reverse transcription-polymerase chain reaction detection of sapoviruses (SaVs). A SaV RNA standard was also created for quantification of the virus. The IAC was included in the screening of environmental samples for SaVs. From August 2010 to December 2011, 51 wastewater samples were collected from five provinces in South Africa. SaVs were found in 72.5?% (37/51) of samples, including four samples where detection was initially inhibited. SaV concentrations ranged from 4.24?×?103 to 1.31?×?106?copies/ml. The IAC successfully identified samples which contained inhibitors and inclusion of an IAC is necessary to ensure the prevalence of SaVs is accurately determined. SaVs are present at high concentrations in wastewater in several provinces of South Africa. This widespread occurrence indicates that SaV circulation in the South African population may be underestimated.