Identification of marker compounds for Japanese Pharmacopoeia non-conforming jujube seeds from Myanmar

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• 作者：Naohiro Oshima (1)
  Kazumasa Zaima (1)
  Hiroyuki Kamakura (1)
  Akane Hamato (2)
  Yutaka Yamamoto (2)
  Dong Hyo Kang (2)
  Tsuguo Yokokura (3)
  Yukihiro Goda (1)
  Takashi Hakamatsuka (1)
  Takuro Maruyama (1)
  
• 关键词：Jujube Seed ; Ziziphus jujuba var. spinosa ; Ziziphus mauritiana ; Purity test ; Oleanolic acid
• 刊名：Journal of Natural Medicines
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：69
• 期：1
• 页码：68-75
• 全文大小：723 KB
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• 作者单位：Naohiro Oshima (1)
  Kazumasa Zaima (1)
  Hiroyuki Kamakura (1)
  Akane Hamato (2)
  Yutaka Yamamoto (2)
  Dong Hyo Kang (2)
  Tsuguo Yokokura (3)
  Yukihiro Goda (1)
  Takashi Hakamatsuka (1)
  Takuro Maruyama (1)
  
  1. Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan
  2. Tochimoto Tenkaido Co., Ltd., Oniya Kaibara-cho, Tamba, Hyogo, 669-3315, Japan
  3. Nippon Funmatsu Yakuhin Co., Ltd., 3-71 Izumi-cho, Yao, Osaka, 581-0813, Japan
  
• ISSN：1861-0293

文摘

Jujube Seed is a crude drug defined as the seed of Ziziphus jujuba Miller var. spinosa Hu ex H.F. Chou (Rhamnaceae) in the Japanese Pharmacopoeia (JP). Most of the jujube seed in the Japanese markets is imported from China, with the rest obtained from other Asian countries. Here we confirmed the botanical origins of jujube seeds from both China and Myanmar by a DNA sequencing analysis. We found that the botanical origins of the crude drugs from China and Myanmar were Z. jujuba and Z. mauritiana, respectively. Although the jujube seed from China conforms to the JP, that from Myanmar does not. A method for discriminating jujube seeds from China and Myanmar using a chemical approach is thus desirable, and here we sought to identify a compound specific to Z. jujuba. Jujuboside A (1) was identified as a compound specific to Z. jujuba. To establish a purity test of Jujube Seed in the JP against Z. mauritiana, we fractionated the extract of Z. mauritiana seeds and identified frangufoline (2) and oleanolic acid (4) as the marker compounds specific to Z. mauritiana. Thin-layer chromatography (TLC) and gas chromatography-mass spectrometry analyses revealed that the latter compound was useful for testing by TLC analysis. The established TLC conditions were as follows: chromatographic support, silica gel; developing solvent, n-hexane:EtOAc:HCOOH?=?10:5:1; developing length, 7?cm; visualization, diluted sulfuric acid; R f value, 0.43 (oleanolic acid).