Genome-wide binding analysis of the transcriptional regulator TrmBL1 in Pyrococcus furiosus

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• 作者：Robert Reichelt ; Antonia Gindner ; Michael Thomm ; Winfried Hausner
• 关键词：Archaea ; Transcription factor ; Transcription regulation ; TrmB ; TrmBL1 ; TGM ; ChIP ; seq
• 刊名：BMC Genomics
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：17
• 期：1
• 全文大小：2,097 KB
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• 作者单位：Robert Reichelt (1)
  Antonia Gindner (1)
  Michael Thomm (1)
  Winfried Hausner (1)
  
  1. Lehrstuhl für Mikrobiologie und Archaeenzentrum, Universität Regensburg, Universitätsstrasse 31, Regensburg, D-93053, Germany
  
• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Several in vitro studies document the function of the transcriptional regulator TrmBL1 of Pyrococcus furiosus. These data indicate that the protein can act as repressor or activator and is mainly involved in transcriptional control of sugar uptake and in the switch between glycolysis and gluconeogenesis. The aim of this study was to complement the in vitro data with an in vivo analysis using ChIP-seq to explore the genome-wide binding profile of TrmBL1 under glycolytic and gluconeogenic growth conditions.