Oxidative damage and antioxidant defence parameters in the Antarctic bivalve Laternula elliptica as biomarkers for pollution impacts

详细信息    查看全文

• 作者：Kathryn N. Lister ; Miles D. Lamare ; David J. Burritt
• 关键词：Antarctica ; Laternula elliptica ; Oxidative stress ; Antioxidant ; Pollutants
• 刊名：Polar Biology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：38
• 期：10
• 页码：1741-1752
• 全文大小：851 KB
• 参考文献：Abele D, Puntarulo S (2004) Formation of reactive species and induction of antioxidant defence systems in polar and temperate marine invertebrates and fish. Comp Biochem Physiol A Mol Integr Physiol 138:405-15. doi:10.-016/?j.?cbpb.-004.-5.-13 CrossRef PubMed
  Ahn IY (1993) Enhanced particle-flux through the biodeposition by the Antarctic suspension-feeding bivalve Laternula elliptica in Marian Cove, King George Island. J Exp Mar Biol Ecol 171:75-0. doi:10.-016/-022-0981(93)90141-a CrossRef
  Ahn I-Y (1994) Ecology of the Antarctic bivalve Laternula elliptica (King and Broderip) in Collins Harbor, King George Island: benthic environment and an adaptive strategy. Mem Natl Inst Polar Res Spec Issue 50:1-0
  Ahn IY, Lee SH, Kim KT, Shim JE, Kim DY (1996) Baseline heavy metal concentrations in the Antarctic clam, Laternula elliptica in Maxwell Bay, King George Island, Antarctica. Mar Pollut Bull 32:592-98. doi:10.-016/-025-326x(95)00247-k CrossRef
  Ahn IY, Cho KW, Choi KS, Seo Y, Shin J (2000) Lipid content and composition of the Antarctic lamellibranch, Laternula elliptica (King & Broderip) (Anomalodesmata : Laternulidae), in King George Island during an austral summer. Polar Biol 23:24-3. doi:10.-007/?s003000050004 CrossRef
  Ahn I, Kang J, Kim K (2001) The effect of body size on metal accumulations in the bivalve Laternula elliptica. Antarct Sci 13:355-62. doi:10.-017/?S095410200100050- CrossRef
  Almeida EA et al (2007) Oxidative stress in Perna perna and other bivalves as indicators of environmental stress in the Brazilian marine environment: Antioxidants, lipid peroxidation and DNA damage. Comp Biochem Physiol A Mol Integr Physiol 146:588-00. doi:10.-016/?j.?cbpa.-006.-2.-40 CrossRef
  Banowetz GM, Dierksen KP, Azevedo MD, Stout R (2004) Microplate quantification of plant leaf superoxide dismutases. Anal Biochem 332:314-20. doi:10.-016/?j.?ab.-004.-6.-15 CrossRef PubMed
  Barry JP, Dayton PK (1988) Current patterns in McMurdo Sound, Antarctica, and their relationship to local biotic communities. Polar Biol 8:367-76CrossRef
  Baussant T et al (2009) Enzymatic and cellular responses in relation to body burden of PAHs in bivalve molluscs: a case study with chronic levels of North Sea and Barents Sea dispersed oil. Mar Pollut Bull 58:1796-807. doi:10.-016/?j.?marpolbul.-009.-8.-07 CrossRef PubMed
  Benedetti M, Martuccio G, Fattorini D, Canapa A, Barucca M, Nigro M, Regoli F (2007) Oxidative and modulatory effects of trace metals on metabolism of polycyclic aromatic hydrocarbons in the Antarctic fish Trematomus bernacchiile. Aquat Toxicol 85:167-75. doi:10.-016/?j.?aquatox.-007.-8.-09 CrossRef PubMed
  Brey T, Mackensen A (1997) Stable isotopes prove shell growth bands in the Antarctic bivalve Laternula elliptica to be formed annually. Polar Biol 17:465-68. doi:10.-007/?s003000050143 CrossRef
  Brogdon WG, Barber AM (1990) Microplate assay of glutathione S-transferase activity for resistance detection in single-mosquito triturates. Comp Biochem Physiol B Biochem Mol Biol 96:339-42. doi:10.-016/-305-0491(90)90385-7 CrossRef
  Burritt DJ (2008) The polycyclic aromatic hydrocarbon phenanthrene causes oxidative stress and alters polyamine metabolism in the aquatic liverwort Riccia fluitans L. Plant Cell Environ 31:1416-431. doi:10.-111/?j.-365-3040.-008.-1846.?x CrossRef PubMed
  Camus L, Jones MB, B?rseth JF, Gr?svik BE, Regoli F, Depledge MH (2002) Total oxyradical scavenging capacity and cell membrane stability of haemocytes of the Arctic scallop, Chlamys islandicus, following benzo[a]pyrene exposure. Mar Environ Res 54:425-30. doi:10.-016/?S0141-1136(02)00140-X CrossRef PubMed
  Camus L et al (2003) Biomarker responses and PAH uptake in Mya truncata following exposure to oil-contaminated sediment in an Arctic fjord (Svalbard). Sci Total Environ 308:221-34. doi:10.-016/?s0048-9697(02)00616-2 CrossRef PubMed
  Chapman PM, Riddle MJ (2003) Missing and needed: polar marine ecotoxicology. Mar Pollut Bull 46:927-28. doi:10.-016/?s0025-326x(03)00252-2 CrossRef PubMed
  Chapman PM, Riddle MJ (2005) Toxic effects of contaminants in polar marine environments. Environ Sci Technol 39:200A-07A. doi:10.-021/?es0532537 CrossRef PubMed
  Conlan KE, Kim SL, Thurber AR, Hendrycks E (2010) Benthic changes at McMurdo Station, Antarctica following local sewage treatment and regional iceberg-mediated productivity decline. Mar Pollut Bull 60:419-32. doi:10.-016/?j.?marpolbul.-009.-0.-09 CrossRef PubMed
  Costantini D, Rowe M, Butler MW, McGraw KJ (2010) From molecules to living systems: historical and contemporary issues in oxidative stress and antioxidant ecology. Funct Ecol 24:950-59. doi:10.-111/?j.-365-2435.-010.-1746.?x CrossRef
  Cribb AE, Leeder JS, Spielberg SP (1989) Use of a microplate reader in an assay of glutathione-reductase using 5,5-dithiobis(2-nitrobenzoic acid). Anal Biochem 183:195-96. doi:10.-016/-003-2
• 作者单位：Kathryn N. Lister (1)
  Miles D. Lamare (2)
  David J. Burritt (1)
  
  1. Department of Botany, University of Otago, 464 Great King St, Dunedin, 9016, New Zealand
  2. Department of Marine Science, University of Otago, 410 Castle St, Dunedin, 9016, New Zealand
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Ecology
  Oceanography
  Microbiology
  Plant Sciences
  Zoology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-2056

文摘

The Antarctic continent is increasingly vulnerable to anthropogenic pollution; however, assessments of the impacts that chemical pollutants have on cold-adapted marine organisms are limited. Oxidative stress (OS) occurs when the rate of reactive oxygen species generation exceeds the scavenging capacity of an organism’s antioxidant (AO) system and is an important unifying feature underlying the toxicity of many chemical contaminants in aquatic organisms. The Antarctic bivalve Laternula elliptica is a widely distributed, infaunal filter-feeding organism. We analysed AO enzyme activities, levels of the molecular antioxidant glutathione, protein carbonylation and lipid peroxidation as OS biomarkers in L. elliptica from contaminant-impacted sites near McMurdo Station and the relatively pristine Cape Evans. The objective was to evaluate the effectiveness of these biomarkers for detecting contaminant stress in this cold-adapted marine invertebrate. The concentrations of total polycyclic aromatic hydrocarbons were quantified as a proxy for contamination and found to be elevated in gonad and muscle tissues from L. elliptica dwelling in contaminated sites. These individuals exhibited a greater degree of OS than those from the reference site, evidenced by increases in oxidative lipid and protein damage, as well as an upregulation of AO defences. Coincidentally, L. elliptica from the contaminated sites were significantly smaller in shell length (1.3-fold) than those from the reference site. Oxidative biomarkers proved to be useful indicators of contamination exposure in the present study and were used to document ongoing biological impacts from historic and current pollution in McMurdo Sound. Keywords Antarctica Laternula elliptica Oxidative stress Antioxidant Pollutants