Applying Hydrophilicity to PS/DVB-Beads Via Introduction of Protected Comonomers
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- 作者：Kristian Lungfiel ; Andreas Seubert
- 关键词：Seeded polymerization ; Inverse size exclusion chromatography ; Reversed phase HPLC ; Scanning electron microscopy ; Polydivinyl benzene ; Hydrophilicity
- 刊名：Chromatographia
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：79
- 期：1-2
- 页码：5-17
- 全文大小：1,657 KB
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Andreas Seubert (1)
1. Faculty of Chemistry, Analytical Chemistry, University of Marburg, Hans-Meerwein-Str. 4, 35043, Marburg, Germany - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Organic Chemistry
Pharmacy
Biochemistry
Plant Sciences
Measurement Science and Instrumentation - 出版者：Vieweg Verlag
- ISSN：1612-1112
文摘
Hydroxyl functionalized polystyrene-co-divinyl benzene beads were prepared via Ugelstads two-step activated swelling procedure as monodisperse particles. The prefunctional monomers p-acetoxystyrene and p-vinylbenzylchloride were used as comonomers with up to 28 % (v/v) of the total monomer load to directly introduce functional groups, which subsequently got hydroxylized. Particle morphology was examined with scanning electron microscopy and porosity by inverse size exclusion chromatography. The particle size ranged from 4.0 to 4.5 µm and porosity was only slightly affected giving mean pore diameters of ≈50 Å and specific surface area of ≈1100 m2/g for all materials. Hydroxylation reaction was monitored by FTIR-spectrometry and for p-vinylbenzylchloride also by XRF spectrometry. While reactivity was good for hydroxylation of p-acetoxystyrene, beads with p-vinylbenzylchloride showed significantly reduced yield in the hydroxylation step. RP-HPLC measurements with a series of homologous alkyl benzenes and acetophenone at different MeCN/H2O ratios gave decreased retention due to increased hydrophilicity of the backbone polymer. Highest hydrophobicity reduction was found for phenol type beads which lost up to 45 % of their RP retention.