Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro

详细信息    查看全文

• 作者：Jingyao Zhang (1)
  Qifei Wu (2)
  Zhe Wang (2)
  Yong Zhang (2)
  Guangjian Zhang (2)
  Junke Fu (2)
  Chang Liu (1)
  
  1. Department of Hepatobiliary Surgery ; the First Affiliated Hospital ; Medical School ; Xi鈥檃n Jiaotong University ; Xi鈥檃n ; China
  2. Department of Thoracic Surgery ; the First Affiliated Hospital ; Medical School ; Xi鈥檃n Jiaotong University ; Yan Ta West Road No. 277 ; Xi鈥檃n ; Shaanxi ; 710061 ; China
  
• 关键词：Cell cycle ; PSF1 ; Lung cancer ; Proliferation
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：36
• 期：3
• 页码：2163-2168
• 全文大小：1,718 KB
• 参考文献：1. Jemal, A, Bray, F, Center, MM, Ferlay, J, Ward, E, Forman, D (2011) Global cancer statistics. CA Cancer J Clin 61: pp. 69-90 CrossRef
  2. Blanco, R, Maestu, I, Torre, MG, Cassinello, A, Nunez, I (2014) A review of the management of elderly patients with non-small-cell lung cancer. Ann Oncol Off J Eur Soc Med Oncol ESMO.
  3. Jemal A, Center MM, DeSantis C, Ward EM. Global patterns of cancer incidence and mortality rates and trends. Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the Am Soc Prevent Oncol. 2010;19(8):1893鈥?07. doi:10.1158/1055-9965.EPI-10-0437 .
  4. Huang, CH, Chang, PM, Lin, YJ, Wang, CH, Huang, CY, Ng, KL (2014) Drug repositioning discovery for early- and late-stage non-small-cell lung cancer. BioMed Res Int 2014: pp. 193817
  5. Reungwetwattana, T, Dy, GK (2013) Targeted therapies in development for non-small cell lung cancer. J Carcinog 12: pp. 22 CrossRef
  6. Nagahama, Y, Ueno, M, Miyamoto, S, Morii, E, Minami, T, Mochizuki, N (2010) PSF1, a DNA replication factor expressed widely in stem and progenitor cells, drives tumorigenic and metastatic properties. Cancer Res 70: pp. 1215-24 CrossRef
  7. Ueno, M, Itoh, M, Kong, L, Sugihara, K, Asano, M, Takakura, N (2005) PSF1 is essential for early embryogenesis in mice. Mol Cell Biol 25: pp. 10528-32 CrossRef
  8. Matsui, T, Kinugasa, Y, Tahara, H, Kanakura, Y, Takakura, N (2013) Possible role of mural cell-covered mature blood vessels in inducing drug resistance in cancer-initiating cells. Am J Pathol 182: pp. 1790-9 CrossRef
  9. Hokka, D, Maniwa, Y, Tane, S, Nishio, W, Yoshimura, M, Okita, Y (2013) Psf3 is a prognostic biomarker in lung adenocarcinoma. Lung Cancer 79: pp. 77-82 CrossRef
  10. Wen, JZ, Han, XY, Wei, B, Zhang, S, Wei, HB (2013) Expression of PSF1 in colon cancer tissues and its effect on the proliferation of colon cancer cells. Zhonghua wei chang wai ke za zhi = Chin J Gastrointest Surg 16: pp. 70-4
  11. Zhou, L, Sun, XJ, Liu, C, Wu, QF, Tai, MH, Wei, JC (2014) Overexpression of PSF1 is correlated with poor prognosis in hepatocellular carcinoma patients. Int J Biol Markers.
  12. Obama, K, Ura, K, Satoh, S, Nakamura, Y, Furukawa, Y (2005) Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma. Oncol Rep 14: pp. 701-6
  13. Pai, CC, Garcia, I, Wang, SW, Cotterill, S, Macneill, SA, Kearsey, SE (2009) GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast. Mol Biol Cell 20: pp. 1213-22 CrossRef
  14. Nagahama, Y, Ueno, M, Haraguchi, N, Mori, M, Takakura, N (2010) PSF3 marks malignant colon cancer and has a role in cancer cell proliferation. Biochem Biophys Res Commun 392: pp. 150-4 CrossRef
  15. Ryu, B, Kim, DS, Deluca, AM, Alani, RM (2007) Comprehensive expression profiling of tumor cell lines identifies molecular signatures of melanoma progression. PLoS One 2: pp. e594 CrossRef
  16. Nakahara, I, Miyamoto, M, Shibata, T, Akashi-Tanaka, S, Kinoshita, T, Mogushi, K (2010) Up-regulation of PSF1 promotes the growth of breast cancer cells. Genes Cells Devoted Mol Cell Mech 15: pp. 1015-24 CrossRef
  17. Elias J, Dimitrio L, Clairambault J, Natalini R. The p53 protein and its molecular network: modelling a missing link between DNA damage and cell fate. Biochimica et biophysica acta. 2014;1844(1 Pt B):232鈥?7. doi:10.1016/j.bbapap.2013.09.019 .
  18. Hong, B, Heuvel, AP, Prabhu, VV, Zhang, S, El-Deiry, WS (2014) Targeting tumor suppressor p53 for cancer therapy: strategies, challenges and opportunities. Curr Drug Targets 15: pp. 80-9 CrossRef
  19. Loayza-Puch, F, Drost, J, Rooijers, K, Lopes, R, Elkon, R, Agami, R (2013) p53 induces transcriptional and translational programs to suppress cell proliferation and growth. Genome Biol 14: pp. R32 CrossRef
  20. Kumar, S (2014) P53 induction accompanying G2/M arrest upon knockdown of tumor suppressor HIC1 in U87MG glioma cells. Mol Cell Biochem 395: pp. 281-90 CrossRef
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Partner of sld five 1 (PSF1) is a member of the heterotetrameric complex termed GINS. Previous studies have shown that PSF1 is unregulated in several cancer and associated with tumor malignant characters. However, the effects of PSF1 in lung cancer are still unclear. The goal of this study was to investigate the effects of PSF1 on the proliferation capacities of lung cancer. To start with, expression of PSF1 in 22 human lung cancer samples and adjacent non-tumor samples were detected by real-time RT-PCR and Western blotting. Our results showed that PSF1 was overexpressed in lung cancer samples compared to adjacent non-tumor samples. To achieve better insights of PSF1 functions in lung cancer cells, we used PSF1-specific small interfering RNA (siRNA) successfully inhibit the expression of PSF1 in messenger RNA (mRNA) and protein levels. In addition, we used lung cancer cell lines with different p53 gene background (p53 null and p53 wild-type). The results showed that knockdown of PSF1 inhibited cell proliferation and caused cell cycle arrest of lung cancer cells in a p53-independent manner. Our data indicated that PSF1 is functionally involved in lung cancer cell proliferation and is a potential target for lung cancer therapy.