Quantification of Antioxidants in Medicinal Plants and Foodstuffs Using Ce(IV) with Indigo Carmine as Chromogenic Probe

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• 作者：Padmarajaiah Nagaraja (1) profpn58@yahoo.com
  Anandamurthy Suma (1)
  Narayana Aradhana (1)
  Anantharaman Shivakumar (1)
  Krishnegowda Avinash (1)
  Honnur Krishna (1)
• 关键词：Antioxidant &#8211 ; Spectrophotometry &#8211 ; Ce(IV) &#8211 ; Indigo Carmine dye &#8211 ; Trolox equivalent antioxidant capacities (TEAC)
• 刊名：Food Analytical Methods
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：5
• 期：4
• 页码：909-919
• 全文大小：312.3 KB
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• 作者单位：1. Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore, 570006 Karnataka, India
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

A simple, novel, sensitive and diversely applicable antioxidant assay method has been developed for the determination of total antioxidant capacity of some foodstuffs and medicinal plants. The method is based on the oxidation of antioxidants by a known amount of Ce(IV) sulphate under slightly acidic medium and subsequently allowing the unreacted Ce(IV) to react with a known amount of Indigo Carmine dye followed by measuring the unreacted blue-coloured Indigo Carmine dye solution at λ _max = 610 nm at room temperature. The antioxidant power was evaluated in terms of trolox equivalent antioxidant capacities and compared with those of ferric-reducing antioxidant power (FRAP–1.10 phenanthroline), Cu(II) reduction assay using bathocuproinedisulfonic acid disodim salt and 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid methods. A good correlation (R 2 = 0.926) was found with the classical spectrophotometric FRAP assay. The proposed method is reproducible and successfully applied to assess the total antioxidant capacities in foodstuffs, vegetables and medicinal plants. The obtained results were compared with reference methods. Interference from other substances was also studied.