Use of the cryptogein gene to stimulate the accumulation of bacopa saponins in transgenic Bacopa monnieri plants

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• 作者：Sukanya Majumdar (1)
  Saraswati Garai (2)
  Sumita Jha (1) sumitajha.cu@gmail.com
• 关键词：Cryptogein &#8211 ; Genetic transformation &#8211 ; Bacopa monnieri &#8211 ; Jujubogenin glycosides
• 刊名：Plant Cell Reports
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：31
• 期：10
• 页码：1899-1909
• 全文大小：572.5 KB
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• 作者单位：1. Department of Botany, Centre of Advanced Study, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019 India2. Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Jadavpur, Kolkata, 700032 India
• ISSN：1432-203X

文摘

Genetic transformation of the Indian medicinal plant, Bacopa monnieri, using a gene encoding cryptogein, a proteinaceous elicitor, via Ri and Ti plasmids, were established and induced bioproduction of bacopa saponins in crypt-transgenic plants were obtained. Transformed roots obtained with A. rhizogenes strain LBA 9402 crypt on selection medium containing kanamycin (100 mg l−1) dedifferentiated forming callus and redifferentiated to roots which, spontaneously showed shoot bud induction. Ri crypt-transformed plants thus obtained showed integration and expression of rol genes as well as crypt gene. Ti crypt-transformed B. monnieri plants were established following transformation with disarmed A. tumefaciens strain harboring crypt. Transgenic plants showed significant enhancement in growth and bacopa saponin content. Bacopasaponin D (1.4–1.69 %) was maximally enhanced in transgenic plants containing crypt. In comparison to Ri-transformed plants, Ri crypt-transformed plants showed significantly (p ≤ 0.05) enhanced accumulation of bacoside A₃, bacopasaponin D, bacopaside II, bacopaside III and bacopaside V. Produced transgenic lines can be used for further research on elicitation in crypt-transgenic plants as well as for large scale production of saponins.