Fingerprint analysis of processed Rhizoma Chuanxiong by high-performance liquid chromatography coupled with diode array detection

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• 作者：Jia-Yan Fang (1)
  Lin Zhu (1)
  Tao Yi (1)
  Jian-Ye Zhang (2)
  Ling Yi (1)
  Zhi-Tao Liang (1)
  Li Xia (3)
  Jia-Fu Feng (4)
  Jun Xu (1)
  Yi-Na Tang (1)
  Zhong-Zhen Zhao (1)
  Hu-Biao Chen (1)
  
  1. School of Chinese Medicine ; Hong Kong Baptist University ; Hong Kong Special Administrative Region ; Hong Kong ; People鈥檚 Republic of China
  2. School of Pharmaceutical Sciences ; Guangzhou Medical University ; Guangzhou ; 510182 ; People鈥檚 Republic of China
  3. School of Traditional Chinese Medicine ; Guangdong Food and Drug Vocational College ; Guangzhou ; 510520 ; People鈥檚 Republic of China
  4. Leshan Pharmaceutical Research Center ; Leshan Vocational & Technical College ; Leshan ; 614000 ; People鈥檚 Republic of China
  
• 关键词：Rhizoma Chuanxiong ; HPLC ; DAD ; Ligusticum chuanxiong ; Fingerprint ; Processing
• 刊名：Chinese Medicine
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：10
• 期：1
• 全文大小：909 KB
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• 刊物主题：Complementary & Alternative Medicine;
• 出版者：BioMed Central
• ISSN：1749-8546

文摘

Background Rhizoma Chuanxiong (RC) is the dried rhizome of Ligusticum chuanxiong Hort., and various types of processed Rhizoma Chuanxiong (PRC) are widely used in China. However, quality assurance and quality control of these processed medicines remain challenging. This study aims to investigate the chemical compositions of various PRC preparations by a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) method. Methods A HPLC-DAD method with validation was developed for PRC samples. Seven batches of plant samples from two processing methods, stir-frying and steaming, were analyzed by the HPLC-DAD method. Common peaks in PRC chromatograms were chosen to calculate their relative retention time (RRT) and relative peak area (RPA), and similarity analyses of the chromatographic fingerprints were conducted by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A). Results In the 24-h stability test, the relative standard deviation for the RRT and RPA was less than 0.07% and 2.57%, respectively. The precision was less than 0.08% for the RRT and 2.48% for the RPA. The repeatability for the RRT and RPA was less than 0.03% and 2.64%, respectively. The similarities between the seven PRC batches were range from 0.956 to 0.990. After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material. Conclusions The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD.