Epigenetic regulation of human hedgehog interacting protein in glioma cell lines and primary tumor samples

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• 作者：Mehdi H. Shahi ; Idoya Zazpe ; Mohammad Afzal ; Subrata Sinha…
• 关键词：Human hedgehog interacting protein (HHIP) ; Glioma ; Glioblastoma promoter methylation ; Melting curve analysis ; Meth (MCA ; Meth) ; GLI1
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：36
• 期：4
• 页码：2383-2391
• 全文大小：1,045 KB
• 参考文献：1.Dahmane N, Ruiz i Altaba A. Sonic hedgehog regulates the growth and patterning of the cerebellum. Development. 1999;126:3089-00.PubMed
  2.Fu M, Lui VC, Sham MH, Pachnis V, Tam PK. Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut. J Cell Biol. 2004;166:673-4.View Article PubMed Central PubMed
  3.Fuccillo M, Joyner AL, Fishell G. Morphogen to mitogen: the multiple roles of hedgehog signalling in vertebrate neural development. Nat Rev Neurosci. 2006;7:772-3.View Article PubMed
  4.Preat T, Therond P, Lamour-Isnard C, Limbourg-Bouchon B, Tricoire H, Erk I, et al. A putative serine/threonine protein kinase encoded by the segment-polarity fused gene of Drosophila. Nature. 1990;347:87-.View Article PubMed
  5.Sisson JC, Ho KS, Suyama K, Scott MP. Costal2, a novel kinesin-related protein in the Hedgehog signaling pathway. Cell. 1997;90:235-5.View Article PubMed
  6.Yoon JW, Kita Y, Frank DJ, Majewski RR, Konicek BA, Nobrega MA, et al. Gene expression profiling leads to identification of GLI1-binding elements in target genes and a role for multiple downstream pathways in GLI1-induced cell transformation. J Biol Chem. 2002;277:5548-5.View Article PubMed
  7.Chuang PT, Kawcak T, McMahon AP. Feedback control of mammalian Hedgehog signaling by the Hedgehog-binding protein, Hip1, modulates Fgf signaling during branching morphogenesis of the lung. Genes Dev. 2003;17:342-.View Article PubMed Central PubMed
  8.Chuang PT, McMahon AP. Vertebrate Hedgehog signalling modulated by induction of a Hedgehog-binding protein. Nature. 1999;397:617-1.View Article PubMed
  9.Martin ST, Sato N, Dhara S, Chang R, Hustinx SR, Abe T, et al. Aberrant methylation of the Human Hedgehog interacting protein (HHIP) gene in pancreatic neoplasms. Cancer Biol Ther. 2005;4:728-3.View Article PubMed
  10.Tada M, Kanai F, Tanaka Y, Tateishi K, Ohta M, Asaoka Y, et al. Down-regulation of hedgehog-interacting protein through genetic and epigenetic alterations in human hepatocellular carcinoma. Clin Cancer Res. 2008;14:3768-6.View Article PubMed
  11.Taniguchi H, Yamamoto H, Akutsu N, Nosho K, Adachi Y, Imai K, et al. Transcriptional silencing of hedgehog-interacting protein by CpG hypermethylation and chromatic structure in human gastrointestinal cancer. J Pathol. 2007;213:131-.View Article PubMed
  12.Shahi MH, Afzal M, Sinha S, Eberhart CG, Rey JA, Fan X, et al. Human hedgehog interacting protein expression and promoter methylation in medulloblastoma cell lines and primary tumor samples. J Neurooncol. 2011;103:287-6.View Article PubMed
  13.Chun SG, Zhou W, Yee NS. Combined targeting of histone deacetylases and hedgehog signaling enhances cytoxicity in pancreatic cancer. Cancer Biol Ther. 2009;8:1328-9.View Article PubMed
  14.Shahi MH, Lorente A, Castresana JS. Hedgehog signalling in medulloblastoma, glioblastoma and neuroblastoma. Oncol Rep. 2008;19:681-.PubMed
  15.Katoh Y, Katoh M. Comparative genomics on HHIP family orthologs. Int J Mol Med. 2006;17:391-.PubMed
  16.Li LC, Dahiya R. MethPrimer: designing primers for methylation PCRs. Bioinformatics. 2002;18:1427-1.View Article PubMed
  17.Lorente A, Mueller W, Urdangarin E, Lazcoz P, von Deimling A, Castresana JS. Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR. BMC Cancer. 2008;8:61.View Article PubMed Central PubMed
  18.Utikal J, Gratchev A, Muller-Molinet I, Oerther S, Kzhyshkowska J, Arens N, et al. The expression of metastasis suppressor MIM/MTSS1 is regulated by DNA methylation. Int J Cancer. 2006;119:2287-3.View Article PubMed
  19.Wang LH, Choi YL, Hua XY, Shin YK, Song YJ, Youn SJ, et al. Increased expression of sonic hedgehog and altered methylation of its promoter region in gastric cancer and its related lesions. Mod Pathol. 2006;19:675-3.View Article PubMed
  20.Fu X, Yang X, Li J, Tian X, Cai J, Zhang Y. Opposite expression patterns of Sonic hedgehog and Indian hedgehog are associated with aberrant methylation status of their promoters in colorectal cancers. Pathology. 2010;42:553-.View Article PubMed
  21.Shahi MH, Schiapparelli P, Afzal M, Sinha S, Rey JA, Castresana JS. Expression and epigenetic modulation of sonic hedgehog-GLI1 pathway genes in neuroblastoma cell lines and tumors. Tumour Biol. 2011;32:113-7.View Article PubMed
  22.Shahi MH, Afzal M, Sinha S, Eberhart CG, Rey JA, Fan X, et al. Regulation of sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 and their epigenetic status in medulloblastoma and astrocytoma. BMC Cancer. 2010;10:614.View Article PubMed Central PubMed
  23.Wang TP, Hsu SH, Feng HC, Huang RF. Folate deprivation enhances invasiveness of human colon cancer cells mediated by activation of sonic hedgehog signaling through promoter hypomethylation and cross action with transcription nuclear factor-kappa B pathway. Carcinogenesis.
• 作者单位：Mehdi H. Shahi (1) (9)
  Idoya Zazpe (2)
  Mohammad Afzal (3)
  Subrata Sinha (4)
  Robert B. Rebhun (5)
  Bárbara Meléndez (6)
  Juan A. Rey (7)
  Javier S. Castresana (1) (8)
  
  1. Brain Tumor Biology Unit, University of Navarra School of Sciences, Pamplona, Spain
  9. Interdisciplinary Brain Research Centre, Faculty of Medicine, Aligarh Muslim University, Aligarh, India
  2. Neurosurgery Service, Hospital of Navarra, Pamplona, Spain
  3. Department of Zoology, Aligarh Muslim University, Aligarh, India
  4. National Brain Research Centre, Manesar, Gurgaon, India
  5. Department of Surgical and Radiological Sciences, University of California Davis School of Veterinary Medicine, Davis, CA, USA
  6. Molecular Pathology Research Unit, Department of Pathology, Virgen de la Salud Hospital, Toledo, Spain
  7. IdiPaz Research Unit, La Paz University Hospital, Madrid, Spain
  8. Department of Biochemistry and Genetics, University of Navarra School of Sciences, Pamplona, Spain
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Glioma constitutes one of the most common groups of brain tumors, and its prognosis is influenced by different genetic and epigenetic modulations. In this study, we demonstrated low or no expression of hedgehog interacting protein (HHIP) in most of the cell lines and primary glioma tumor samples. We further proceeded to promoter methylation study of this gene in the same cell lines and primary tumor samples and found 87?% (7/8) HHIP methylation in glioblastoma cell lines and 75?% (33/44) in primary tumor samples. These methylation pattern correlates with low or unexpressed HHIP in both cell lines and primary tumor samples. Our results suggest the possibility of epigenetic regulation of this gene in glioma, similarly to medulloblastoma, gastric, hepatic, and pancreatic cancers. Also, HHIP might be a diagnostic or prognostic marker in glioma and help to the detection of these tumors in early stages of disease.