Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation

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• 作者：H. H. Wang ; Q. Cui ; T. Zhang ; Z. B. Wang ; Y. C. Ouyang…
• 刊名：Histochemistry and Cell Biology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：145
• 期：6
• 页码：647-657
• 全文大小：5,901 KB
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Anatomy
  Medicine/Public Health, general
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-119X
• 卷排序：145

文摘

Rab family members play important roles in membrane trafficking, cell growth, and differentiation. Almost all components of the cell endomembrane system, the nucleus, and the plasma membrane are closely related to RAB proteins. In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35, in mouse oocyte meiotic maturation and activation. The three Rab family members showed different localization patterns in oocytes. Microinjection of siRNA, antibody injection, or inhibitor treatment showed that (1) Rab3A regulates peripheral spindle and cortical granule (CG) migration, polarity establishment, and asymmetric division; (2) Rab27A regulates CG exocytosis following MII-stage oocyte activation; and (3) Rab35 plays an important role in spindle organization and morphology maintenance, and thus meiotic nuclear maturation. These results show that Rab proteins play important roles in mouse oocyte meiotic maturation and activation and that different members exert different distinct functions.KeywordsMeiotic maturationCortical granulesRab3ARab27ARab35