Reversible S-nitrosylation limits over synthesis of fungal styrylpyrone upon nitric oxide burst

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• 作者：Yanxia Zhao ; Meihong He ; Qi Xi ; Jianing Ding…
• 关键词：Inonotus obliquus ; Styrylpyrone biosynthesis ; S ; nitrosylation ; Denitrosylation
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：100
• 期：9
• 页码：4123-4134
• 全文大小：850 KB
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• 作者单位：Yanxia Zhao (1)
  Meihong He (1)
  Qi Xi (1)
  Jianing Ding (1)
  Baixia Hao (1)
  Nancy P Keller (2)
  Weifa Zheng (1)
  
  1. Laboratory of Biotechnology on Medicinal Plants, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China
  2. Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, WI, 53706, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Nitric oxide (NO) is known to be involved in modulating production of styrylpyrone polyphenols in the basidiomycete Inonotus obliquus. However, it remains unknown how NO orchestrates fungal styrylpyrone biosynthesis. Here, we show that a transient NO burst correlated with an enhanced expression of phenylalanine ammonia lyase (PAL), 4-coumarate CoA ligase (4CL), and styrylpyrone synthase (SPS), the key enzymes involved in styrylpyrone biosynthesis, and subsequently an increased production of styrylpyrone polyphenols. In parallel, the NO burst also resulted in S-nitrosylation of PAL, 4CL, and SPS, which compromised their enzymatic activities mediating a post-translational feedback mechanism that keeps NO-dependent transcriptional activation in check. Moreover, dysfunction of thioredoxin reductase (TrxR) further increased the formation of S-nitrosylated proteins, implicating the significance of the Trx system in maintaining a low level of protein-nitrosothiols. Three thioredoxin-like proteins (TrxLs) from I. obliquus show in vitro denitrosylation potential toward S-nitrosylated proteins via trans-denitrosylation or mixed disulfide intermediates. Thus, S-nitrosylation triggered by the NO burst limits over production of fungal styrylpyrone polyphenols, and denitrosylation by TrxLs that act in concert with TrxR play a key role in maintaining redox balance and orchestrating catalytic activities of the enzymes engaged in styrylpyrone synthetic metabolism.