Pyrosequencing reveals bacterial community differences in composting and vermicomposting on the stabilization of mixed sewage sludge and cattle dung

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• 作者：Baoyi Lv ; Meiyan Xing ; Jian Yang ; Liangbo Zhang
• 关键词：Pyrosequencing ; Bacterial community ; Composting ; Vermicomposting ; Sewage sludge ; Cattle dung
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：99
• 期：24
• 页码：10703-10712
• 全文大小：1,216 KB
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• 作者单位：Baoyi Lv (1) (2)
  Meiyan Xing (2)
  Jian Yang (2)
  Liangbo Zhang (3)
  
  1. College of Ocean Science and Engineering, Shanghai Maritime University, Shanghai, 201306, China
  2. Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China
  3. College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

This study aimed to compare the microbial community structures and compositions in composting and vermicomposting processes. We applied 454 high-throughput pyrosequencing to analyze the 16S rRNA gene of bacteria obtained from bio-stabilization of sewage sludge and cattle dung. Results demonstrated that vermicomposting process presented higher operational taxonomic units and bacterial diversity than the composting. Analysis using weighted UniFrac indicated that composting exhibited higher effects on shaping microbial community structure than the vermicomposting. The succession of dominant bacteria was also detected during composting. Firmicutes was the dominant bacteria in the thermophilic phase of composting and shifted to Actinomycetes in the maturing stage. By contrast, Proteobacteria accounted for the highest proportions in the whole process of the vermicomposting. Furthermore, vermicomposting contained more uncultured and unidentified bacteria at the taxonomy level of genus than the composting. In summary, the bacterial community during composting significantly differed from that during vermicomposting. These two techniques played different roles in changing the diversity and composition of microbial communities. Keywords Pyrosequencing Bacterial community Composting Vermicomposting Sewage sludge Cattle dung