In vitro plant regeneration from cotyledonary explants of Cucumis melo L. var. cantalupensis and genetic stability evaluation using RAPD analysis

详细信息    查看全文

• 作者：Maria Silvia Sebastiani ; Nadia Ficcadenti
• 关键词：Melon ; Doubled ; haploid lines ; In vitro regeneration ; RAPD ; Genetic homogeneity
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：124
• 期：1
• 页码：69-79
• 全文大小：1,656 KB
• 参考文献：Aggarwal D, Kumar A, Reddy MS (2010) Shoot organogenesis in elite clones of Eucalyptus tereticornis. Plant Cell Tiss Org Cult 102:45鈥?2CrossRef
  Akin-Idowu PE, Ibitoye DO, Ademoyegun OT (2009) Tissue culture as a plant production technique for horticultural crops. Afr J Biotechnol 8(16):3782鈥?788
  Biswas MK, Dutt M, Roy UK, Islam R, Hossain M (2009) Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Sci Hortic 122:409鈥?16CrossRef
  Cheruvathur MK, Abraham J, Thomas TD (2013) Plant regeneration through callus organogenesis and true-to-type conformity of plants by RAPD analysis in Desmodium gangeticum (Linn.) DC. Appl Biochem Biotechnol 169:1799鈥?810CrossRef PubMed
  Choi JY, Shin JS, Chung YS, Hyung N (2012) An efficient selection and regeneration protocol for Agrobacterium-mediated transformation of oriental melon (Cucumis melo L. var. makuwa). Plant Cell Tiss Org Cult 110:133鈥?40CrossRef
  Chovelon V, Restier V, Giovinazzo N, Dogimont C, Aarrouf J (2011) Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants? Plant Cell Rep 30:2001鈥?011CrossRef PubMed
  Curuk S, Elman C, Schlarman E, Sagee O, Shomer I, Cetiner S, Gray DJ, Gaba V (2002) A novel pathway for rapid shoot regeneration from the proximal zone of the hypocotyl of melon (Cucumis melo L.). In Vitro Cell Dev Biol Plant 38:260鈥?67CrossRef
  Curuk S, Ananthakrishnan G, Singer S, Xia X, Elman C, Nestel D, Cetiner S, Gaba V (2003) Regeneration in vitro from the hypocotyls of Cucumis species produces almost exclusively diploid shoots, and does not require light. HortSci 38:105鈥?09
  Devi KD, Punyarani K, Singh NS, Devi HS (2013) An efficient protocol for total DNA extraction from the members of order Zingiberales: suitable for diverse PCR based downstream applications. Springer Plus 2:669PubMedCentral CrossRef PubMed
  Dirks R, van Buggenum M (1989) In vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L. Plant Cell Rep 7:626鈥?27CrossRef PubMed
  Dolezzel J, Bartos J (2005) Plant DNA flow cytometry and estimation of nuclear genome size. Ann Bot 95:99鈥?10CrossRef
  Ezura H, Osawa K (1994) Ploidy of somatic embryos and the ability to regenerate plantlets in melon (Cucumis melo L). Plant Cell Rep 14:107鈥?11PubMed
  Ezura H, Amagai H, Yoshioka K, Oosawa K (1992) Highly frequent appearance of tetraploidy in regenerated plants, a universal phenomenon in tissue culture melon (Cucumis melo L). Plant Sci 85:209鈥?13CrossRef
  Ezura H, Amagai H, Kikuta I, Kubota M, Oosawa K (1995) Selection of somaclonal variants with low-temperature germinability in melon (Cucumis melo L). Plant Cell Rep 14:684鈥?88CrossRef PubMed
  Ficcadenti N, Rotino G (1995) Genotype and medium affect shoot regeneration of melon. Plant Cell Tiss Org Cult 40:293鈥?95CrossRef
  Ficcadenti N, Veronese P, Sestili S, Crino P, Lucretti S, Schiavi M (1995) Influence of genotype on the induction of haploidy in Cucumis melo L. by using irradiated pollen. J Genet Breed 49:359鈥?64
  Garcia-Mas J, Benjak A, Sanseverino W, Bourgeois M, Mir G, Gonz谩lez VM, H茅naff E, C芒mara F, Cozzuto L, Lowy E, Alioto T, Capella-Guti茅rrez S, Blanca J, Ca帽izares J, Ziarsolo P, Gonzalez-Ibeas D, Rodr铆guez-Moreno L, Droege M, Du L, Alvarez-Tejado M, Lorente-Galdos B, Mel茅 M, Yang L, Weng Y, Navarro A, Marques-Bonet T, Aranda MA, Nuez F, Pic贸 B, Gabald贸n T, Roma G, Guig贸 R, Casacuberta JM, Ar煤s P, Puigdom猫nech P (2012) The genome of melon (Cucumis melo L.). Proc Natl Acad Sci USA 109(29):11872鈥?1877
  Govind KR, Major S, Neha PR, Bhardwaj DR, Sanjeev K (2012) In vitro propagation of spine gourd (Momordica dioica Roxb.) and assessment of genetic fidelity of micropropagated plants using RAPD analysis. Physiol Mol Biol Plants 18(3):273鈥?80CrossRef
  Guis M, Ben-Amor M, Latche A, Peche JC, Roustan JP (2000) A reliable system for the transformation of cantaloupe Charentais melon (Cucumis melo L. var. cantaloupensis) leading to a majority of diploid regenerants. Sci Hort 84:91鈥?9CrossRef
  Jain R, Sinha A, Jain D, Kachhwaha S, Kothari SL (2011) Adventitious shoot regeneration and in vitro biosynthesis of steroidal lactones in Withania coagulans (stock) Dunal. Plant Cell Tiss Org Cult 105:135鈥?40CrossRef
  Jeyakumar JJ, Kamaraj M, Thiruvengadam M (2014) Efficient plant regeneration from petiole explants of West Indian gherkin (Cucumis anguria L.) via indirect organogenesis. J Plant Biochem Biotechnol 23(3):307鈥?15CrossRef
  Jikku J (2013) Studies on the induction of variation through in vitro culture in Jatropha curcas L. Ph.D. thesis dissertation. Cochin University of Science and Technology, Cochin
  Jin S, Mushke R, Zhu H, Tu L, Lin Z, Zhang Y, Zhang X (2008) Detection of somaclonal variation of cotton (Gossypium hirsutum) using cytogenetics, flow cytometry and molecular markers. Plant Cell Rep 27:1303鈥?316CrossRef PubMed
  Kathal R, Bhatnagar SP, Bhojwani SS (1994) Plant regeneration from the callus derived from roots explants of Cucumis melo L. cultivar 鈥淧usa Sharbati鈥? Plant Sci 96:137鈥?42CrossRef
  Kintzios S, Sereti E, Bluchos P, Drossopoulos JB, Kitsaki CK, Liopa- Tsakalidis A (2002) Growth regulator pre-treatment improves somatic embryogenesis from leaves of squash (Cucurbita pepo L.) and melon (Cucumis melo L.). Plant Cell Rep 21:1鈥?CrossRef
  Krishnan SR, Priya AM, Ramesh M (2013) Rapid regeneration and ploidy stability of 鈥榗v IR36鈥?indica rice (Oryza Sativa L.) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation. Bot Stud 54:47CrossRef
  Kumar N, Vijayanand KG, Reddy MP (2011) Plant regenerationin non-toxic Jatropha curcas: impacts of plant growth regulators, source and type of explants. J Plant Biochem Biotechnol 20:125鈥?33CrossRef
  Larkin PJ, Scowcroft WR (1981) Somaclonal variation-a novel source of variability from plant cell cultures for plant improvement. Theor Appl Genet 60:197鈥?14CrossRef PubMed
  Liborio LC, Januzzi BM, Stefano SMD, Martinelli AP (2001) In vitro morphogenesis of Cucumis melo var. inodorus. Plant Cell Tiss Org Cult 65:81鈥?9CrossRef
  Liu L, Kakihara F, Kato M (2004) Characterization of six varieties of Cucumis melo L. based on morphological and physiological characters, including shelf-life of fruit. Euphytica 135:305鈥?13CrossRef
  Mallon R, Rodriguez-Oubina J, Luz Gonzalez M (2010) In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis. Plant Cell Tiss Org Cult 101:31鈥?9CrossRef
  Monforte AJ, Oliver M, Gonzalo MJ, Alvarez JM, Dolcet-Sanjuan R, Arus P (2004) Identification of quantitative trait loci involved in fruit quality traits in melon (Cucumis melo L.). Theor Appl Genet 108:750鈥?58CrossRef PubMed
  Moon JG, Choo BK, Hs D, Kwon TH, Yang MS, Ryu JH (2000) Effects of growth regulators on plant regeneration from the cotyledon explant in oriental melon (Cucumis melo L.). Kor J Plant Tissue Cult 27:1鈥?
  Moreno V, Garcia-Sogo M, Granell I, Garcia-Sogo B, Roig LA (1985) Plant regeneration from calli of melon (Cucumis melo L. cv. Amarillo Oro). Plant Cell Tiss Org Cult 5:139鈥?46CrossRef
  Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473鈥?97CrossRef
  Nunez-Palenius HG, Cantliffe DJ, Huber DJ, Ciardi J, Klee HJ (2006) Transformation of a muskmelon 鈥楪alia鈥?hybrid parental line (Cucumis melo L. var. reticulatus Ser.) with an antisense ACC oxidase gene. Plant Cell Rep 25:198鈥?05CrossRef PubMed
  Nunez-Palenius HG, Gomez-Lim M, Ochoa-Alejo N, Grumet R, Lester G, Cantliffe DJ (2008) Melon fruits: genetic diversity, physiology, and biotechnology features. Crit Rev Biotechnol 28:13鈥?5CrossRef PubMed
  Otieno S, Muthoni J, Mbiyu M, Lung鈥檃ho C, Onditi J, Oyoo J (2013) Application of biotechnological tools in potato breeding: current situation and future prospects in Kenya. Int J Biotechnol Res 1(5):071鈥?80
  Pandey RN, Singh SP, Rastogi J, Sharma ML, Singh RK (2012) Early assessment of genetic fidelity in sugarcane (Saccharum officinarum) plantlets regenerated through direct organogenesis with RAPD and SSR markers. Aust J Crop Sci 6:618鈥?24
  Qin Y, Gao LH, Pulli S, Guo YD (2006) Shoot differentiation, regeneration of cauliflower and analysis of somaclonal variation by RAPD. Hereditas 143:91鈥?8CrossRef PubMed
  Ramakrishnan M, Ceasar SA, Duraipandiyan V, Ignacimuthu S (2014) Efficient plant regeneration from shoot apex explants of maize (Zea mays) and analysis of genetic fidelity of regenerated plants by ISSR markers. Plant Cell Tiss Organ Cult 119:183鈥?96CrossRef
  Rani V, Raina S (2000) Genetic fidelity of organized meristem-derived micropropagated plants: a critical reappraisal. In Vitro Cell Dev Biol Plant 36:319鈥?30CrossRef
  Rani V, Parida A, Raina S (1995) Random amplified polymorphicDNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoids Marsh. Plant Cell Rep 14:459鈥?62CrossRef PubMed
  Ren Y, Bang H, Gould J, Rathore K, Patil B, Crosby K (2013) Shoot regeneration and ploidy variation in tissue culture of honeydew melon (Cucumis melo L. inodorus). Vitro Cell Dev Biol Plant 49(2):223CrossRef
  Roy AR, Sajeev S, Pattanayak A, Deka BC (2012) TDZ induced micropropagation in Cymbidium giganteum Wall. Ex Lindl and assessment of genetic variation in the regenerated plants. Plant Growth Regul 68:435鈥?45CrossRef
  Saha S, Sengupta C, Ghosh P (2014a) Evaluation of the genetic fidelity of in vitro propagated Ocimum basilicum L. using RAPD and ISSR markers. J Crop Sci Biotech 17(4):281鈥?87CrossRef
  Saha S, Sengupta C, Roy S, Ghosh P (2014b) Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims. Ind J Plant Physiol 19(2):174鈥?83CrossRef
  Sangeetha P, Venkatachalam P (2014) Induction of direct shoot organogenesis and in vitro flowering from shoot tip explants of cucumber (Cucumis sativus L. cv. Green long). In Vitro Cell Dev Biol Plant 50:242鈥?48CrossRef
  Sebastiani MS, Ficcadenti N (2013) An efficient protocol for in vitro plant regeneration from cotyledon explants of different melon genotypes (Cucumis melo L. var. cantalupensis). Abstract book: Young Researches in Life Science (YRLS) conference, Paris, May 22鈥?4, p.129
  Sheidai M, Aminpoor H, Noormohammadi Z, Farahani F (2008) RAPD analysis of somaclonal variation in banana (Musa acuminata L.) cultivar Valery. Acta Biologica Szegediensis 52(2):307鈥?11
  Skoog F, Miller C (1956) Chemical regulation of growth and organ formation in plant tissues cultivated in vitro. Symp Soc Exp Bot 9:118鈥?31
  Slazak B, Sliwinska E, Sa艂uga M, Ronikier M, Bujak J, S艂omka A, G枚ransson U, Kuta E (2015) Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis. Plant Cell Tiss Org Cult 120(1):179鈥?90CrossRef
  Su YH, Liu YB, Zhang XS (2011) Auxin鈥揷ytokinin interaction regulates meristem development. Mol Plant 4:616鈥?25PubMedCentral CrossRef PubMed
  Sultana RS, Mahabubur RMD (2014) Melon crops improvement through biotechnological techniques for the changing climatic conditions of the 21st century. Int J Genet Genom 2(3):30鈥?1CrossRef
  Tiwari JK, Chandel P, Gupta S, Gopal J, Singh BP (2013) Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers. Physiol Mol Biol Plants 19(4):587鈥?95PubMedCentral CrossRef PubMed
  Xing Y, Yu Y, Luo X, Zhang JN, Zhao B, Guo YD (2010) High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena. Biol Plant 54(2):231鈥?36CrossRef
  Yadav RC, Salah MT, Grumet R (1996) High frequency shoot regeneration from leaf explants of muskmelon. Plant Cell Tiss Org Cult 45:207鈥?14CrossRef
  Yang XM, An LZ, Xiong YC, Zhang JP, Li Y, Xu SJ (2008) Somatic embryogenesis from immature zygotic embryos and monitoring the genetic fidelity of regenerated plants in grapevine. Biol Plant 52(2):209鈥?14CrossRef
  Zhang H, Peng G, Feishi L (2011) Efficient plant regeneration from cotyledonary node explants of Cucumis melo L. Afr J Biotechnol 10(35):6757鈥?761
  Zonneveld BJM, Leitch IJ, Bennett MD (2005) First Nuclear DNA Amounts in more than 300 Angiosperms. Ann Bot 96:229鈥?44PubMedCentral CrossRef PubMed
  
• 作者单位：Maria Silvia Sebastiani (1)
  Nadia Ficcadenti (1)
  
  1. Consiglio per la Ricerca in Agricoltura e l鈥橝nalisi dell鈥橢conomia Agraria (CREA), Unit脿 di Ricerca per l鈥橭rticoltura, Via Salaria 1, 63077, Monsampolo Del Tronto, Italy
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

An efficient plant regeneration protocol useful in genetic studies for important breeding lines of cantaloupe melons was developed. Three cultivars (Charentais-T, Vedrantais and Isabelle) and three doubled-haploid melon lines (NAD, DH-L2 and DH-L6) were tested for their potential to regenerate under in vitro conditions. Cotyledon explants from 6-day-old seedlings were cultured on three Murashige and Skoog based medium supplemented with different combinations of 6-benzylaminopurine (BAP) and indoleacetic acid (IAA). After 5 weeks of culture all the genotypes were able to regenerate on all media, however the regeneration frequency varied widely among the cultivars and the medium combinations used. The Charentais-T cultivar developed the highest number of shoots/explant on media containing BAP 1.0-IAA 1.0 脳 10鈭? mg/L (4.1) and BAP 1.2-IAA 1.2 脳 10鈭? mg/L (4.4) while on medium supplemented with BAP 1.3-IAA 1.3 脳 10鈭? mg/L the Vedrantais genotype gave the best result (2.4). The work first highlighted a good morphogenetic response of the DH lines; among all the genotypes and combination used, NAD (65.6 %) and DH-L6 (85.4 and 50.0 %) had the highest yield of regenerated plants. The results confirmed that the regeneration competence was strongly affected by genotype. Plantlets with well-developed shoot and root systems were successfully acclimatized and exhibited normal morphology and growth characteristics. In order to confirm genetic stability, a PCR-based RAPD analysis was carried out using 20 decamer-primers. No polymorphic bands were observed among all the plants revealing the genetic homogeneity of the regenerants. Furthermore, the ploidy level of regenerated plants was confirmed by flow cytometry. Keywords Melon Doubled-haploid lines In vitro regeneration RAPD Genetic homogeneity