Carbon/nitrogen ratio as a major factor for predicting the effects of organic wastes on soil bacterial communities assessed by DNA-based molecular techniques

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• 作者：Yuan Ge ; Chengrong Chen ; Zhihong Xu ; Simon M. Eldridge ; Kwong Yin Chan ; Yan He and Ji-Zheng He
• 关键词：Bacterial community ; Bacterial diversity ; Cloning and sequencing ; Denaturing gradient gel electrophoresis ; Organic waste ; Soil
• 刊名：Environmental Science and Pollution Research
• 出版年：2010
• 出版时间：March, 2010
• 年：2010
• 卷：17
• 期：3
• 页码：807-815
• 全文大小：210.0 KB

文摘

Background, aim, and scope Current Australian legislation permits the beneficial application of grease trap waste (GTW) to agricultural soil, viewing it as a beneficial source of organic matter and soil conditioner containing no/low amounts of metals or pathogenic organisms. However, little is known about the influence of GTW on soil bacterial community. A field experiment was established at Menangle in south western Sydney in Australia to quantitatively assess the impacts of different types (GTW CO and GTW CL) and amounts of GTW application on the soil bacterial community and diversity. Furthermore, a municipal solid waste (MSW) compost was simultaneously examined to compare against the other organic wastes. Knowledge about the shifts in microbial community structure and diversity following the applications of organic wastes could help to evaluate the ecological consequences on the soil and thus to develop sound regulatory guidelines for the beneficial reuse of organic wastes in agricultural lands.