Serum microRNAs levels in primary focal segmental glomerulosclerosis

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• 作者：Xiaoyi Cai (1)
  Zhengkun Xia (1)
  Chunni Zhang (2)
  Yang Luo (2)
  Yuanfu Gao (1)
  Zhongmin Fan (1)
  Mengyuan Liu (1)
  Ying Zhang (1)
  
• 关键词：Focal segmental glomerulosclerosis ; Minimal change disease ; Nephrotic syndrome ; miRNAs
• 刊名：Pediatric Nephrology
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：28
• 期：9
• 页码：1797-1801
• 全文大小：183KB
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• 作者单位：Xiaoyi Cai (1)
  Zhengkun Xia (1)
  Chunni Zhang (2)
  Yang Luo (2)
  Yuanfu Gao (1)
  Zhongmin Fan (1)
  Mengyuan Liu (1)
  Ying Zhang (1)
  
  1. Department of Pediatric Nephrology, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, Jiangsu Province, China
  2. Department of Clinical Laboratory, Jinling Hospital, Clinical School of Medical College, Nanjing University, 210002, Nanjing, People’s Republic of China
  

文摘

Background MicroRNAs (miRNAs, miRs) are involved in most physiological, developmental, and pathological processes. miR-192 and miR-205 are expressed preferentially in the renal cortex and closely relevant to the renal cell biology. In the present study, we aim to measure the serum levels of miR-192 and miR-205 and their correlation with clinicopathological data in patients with primary focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD). Methods Fifty-six patients (35 male, 21 female) with idiopathic nephrotic syndrome (FSGS 30, MCD 26) and 20 healthy controls were enrolled in the study. We quantified the serum levels of miR-192 and miR-205 in patients with FSGS and MCD by RT-qPCR. Results Patients with FSGS had higher serum levels of miR-192 and miR-205 than those with MCD (324.49?±-2.74 fmol/l versus 90.19?±-7.14 fmol/l, p-lt;-.01, 2.25?±-.69 fmol/l versus 0.60?±-.51 fmol/l, p-lt;-.01, respectively). The level of miR-192 was positively correlated with the proteinuria in patients with FSGS and MCD (r--.62, p-lt;-.001, r--.84, p-lt;-.001, respectively). Similarly, the level of miR-205 was positively correlated with the proteinuria in patients with FSGS (r--.54, p--.002). In addition, the serum level of miR-192 was significantly correlated with the degree of interstitial fibrosis in patients with FSGS (r--.342, p-lt;-.05). Conclusions miR-192 and miR-205 have the potential as markers to differentiate FSGS from MCD.