Scaffold-free and scaffold-assisted 3D culture enhances differentiation of bone marrow stromal cells

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• 作者：Prasanna Vidyasekar ; Pavithra Shyamsunder…
• 关键词：3D culture ; Mesenchymal stem cells ; Differentiation ; Adipogenesis ; RCCS
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：52
• 期：2
• 页码：204-217
• 全文大小：6,050 KB
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• 作者单位：Prasanna Vidyasekar (1)
  Pavithra Shyamsunder (1)
  Sanjeeb Kumar Sahoo (2)
  Rama Shanker Verma (1) (3)
  
  1. Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600 036, India
  2. Institute of Life Sciences, Nalco Square, Bhubaneswar, 751023, India
  3. Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600036, Tamil Nadu, India
  
• 刊物主题：Cell Biology; Developmental Biology; Stem Cells; Cell Culture; Animal Genetics and Genomics;
• 出版者：Springer US
• ISSN：1543-706X

文摘

3D cultures of stem cells can preserve differentiation potential or increase the efficiency of methods that induce differentiation. Mouse bone marrow-derived stromal cells (BMSCs) were cultured in 3D as scaffold-free spheroids or “mesoid bodies” (MBs) and as aggregates on poly(lactic) acid microspheres (MB/MS). 3D cultures demonstrated viable cells, interaction on multiple planes, altered cell morphology, and the formation of structures similar to epithelial cell bridges. Cell proliferation was limited in suspension cultures of MB and MB/MS; however, cells regained proliferative capacity when transferred to flat substrates of tissue culture plates (TCPs). Expanded as monolayer, cells retained expression of Sca-1 and CD44 stem cell markers. 3D cultures demonstrated enhanced potential for adipogenic and osteogenic differentiation showing higher triglyceride accumulation and robust mineralization in comparison with TCP cultures. Enhanced and efficient adipogenesis was also observed in 3D cultures generated in a rotating cell culture system. Preservation of multilineage potential of BMSC was demonstrated in 5-azacytidine treatment of 3D cultures and TCP by expression of cardiac markers GATA4 and ACTA1 although functioning cardiomyocytes were not derived. Keywords 3D culture Mesenchymal stem cells Differentiation Adipogenesis RCCS