Expression of metallothionein protein relating to proliferative cell index in malignant feline mammary tumors using high throughput tissue microarray technique

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• 作者：Anudep Rungsipipat ; Panchan Sitthicharoenchai…
• 关键词：Feline mammary tumor ; Immunohistochemistry ; Metallothionein ; Tissue microarray
• 刊名：Comparative Clinical Pathology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：25
• 期：2
• 页码：449-457
• 全文大小：3,304 KB
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• 作者单位：Anudep Rungsipipat (1)
  Panchan Sitthicharoenchai (2)
  Phimonrat Marlow (2)
  Perapux Prutthithaworn (2)
  Sirikachon Tangkawattana (3)
  
  1. Companion Animal Cancer Research Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand
  2. Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand
  3. Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Pathology
  Hematology
  Oncology
  
• 出版者：Springer London
• ISSN：1618-565X

文摘

The present study aimed to examine the relationship between proliferation markers by detecting Ki-67 and proliferative cell nuclear antigen (PCNA) indices, and metallothionein expression, in different histological groups and nuclear grades of malignant feline mammary tumors by using immunohistochemistry and tissue microarray technique. Seventy feline mammary carcinoma (FMC) biopsy specimens were collected to perform tissue microarray (TMA) slide. The immunohistochemical staining was performed on the TMA slides against pancytokeratin (CK19), PCNA, Ki-67 clone MIB, and metallothionein 1 (MT-1) antibodies. The PCNA index showed no significant differences among histological types and nuclear grades, whereas the Ki-67 index showed statistical difference among histological types and malignancy grades (p < 0.05). The pattern of cytoplasmic MT expression was observed in glandular carcinoma cells. The percentage ± SD of MT expression was 44.13 ± 30.24 in tubular adenocarcinomas, 39.29 ± 26.21 in papillary adenocarcinomas, 22.66 ± 17.81 in cribriform carcinomas, and 38.12 ± 30.33 in solid carcinomas. The percentage ± SD of MT expression was 32.68 ± 27.59 in grade I, 37.34 ± 26.37 in grade II, and 43.54 ± 32.97 in grade III. Our study showed that the expression of MT increased with nuclear grade rather than histological subtype. It is suggested that the role of MT in carcinogenesis and tumor progression could be linked to involvement of MT in processes of cell proliferation and differentiation of feline mammary carcinomas.